中国水稻科学2018，Vol.32Issue(2)：119-127,9.DOI:10.16819/j.1001-7216.2018.7112
利用CRISPR/Cas9技术编辑粒长基因GS3改善粳稻花时
CRISPR/Cas9-mediated Editing of GS3 to Improve Flowering Time in japonica Rice
摘要
Abstract
[Objective]Flowering time asynchrony is one of the main factors limiting seed production of hybrid rice between male and female parents, and some studies have shown that flowering time is related to grain shape. Loss of function mutant of GS3 was used to study the difference of flowering time in this study so as to elucidate the impact of grain shape on flowering time. [Method] CRISPR/Cas9-mediated editing of GS3 to achieve 13 pairs of near-isogenic line japonica varieties.Then the flowering time was investigated with visual methods. [Result] T1individuals derived from T0generation were genotyped. The sequencing results show that some bases were mutated with single base insertion, including Changbai 25, Jijing 102, Zhejing 88, Wuyunjing 27, J42 and others with bases deletion, including Kenjiandao 6, Kongyu 131, Zhejing 22, Yangjing 4227, Nanjing 9108, J5933, J6167, J5938. For the grain shape of T1, the gs3 mutants conferred longer grain length compared to the wild type,and the gs3 mutants of Jijing 102,Kongyu 131, Zhejing 88, Wuyunjing 27 and Yangjing 4227 were significantly earlier in flowering time than those of the wild type. The others were also earlier,but not significant.[Conclusion] Long-grain japonica rice has an earlier flowering time than short-grain japonica rice,which could provide references to study the grain-type breeding of japonica rice,and it would accelerate the breeding process of long-grain japonica parents and promote the development of hybrid japonica rice.关键词
粳稻/花时/CRISPR/Cas9/GS3Key words
japonica rice/flowering time/CRISPR/Cas9/GS3分类
生物科学引用本文复制引用
孟帅,徐鹏,张迎信,王宏,曹立勇,程式华,沈希宏..利用CRISPR/Cas9技术编辑粒长基因GS3改善粳稻花时[J].中国水稻科学,2018,32(2):119-127,9.基金项目
国家863计划资助项目(2014AA10A603) （2014AA10A603）
国家公益性农业科技研究专项(201403002) （201403002）
中国农业科学院创新工程超级稻育种创新团队和水稻杂种优势研究机理研究创新团队资助项目(CAAS-ASTIP-2013-CNRRI) （CAAS-ASTIP-2013-CNRRI）
国家自然科学青年科学基金资助项目(31501290). （31501290）
