中国水稻科学2018，Vol.32Issue(2)：128-136,9.DOI:10.16819/j.1001-7216.2018.7045
低温条件下喷施乙烯利对温敏核不育水稻育性的影响
Effects of Exogenous Ethephon on Fertility of Thermo-sensitive Genic Male Sterile Rice Lines Under Low Temperature Inducement
摘要
Abstract
[Objective]This research aims to elucidate the mechanism behind sterility conversion(infertile convert into fertile) in low temperature of thermo-sensitive genic male sterile rice in seed production in South China by applying exogenous ethephon.[Method]The thermo-sensitive genic male sterile rice cultivars Zhu 1S and Zhun S were treated with artificial cooling water tank (low temperature, 22.5℃) induction for 7 days and sprayed with ethephon (which promote synthesis of ethylene) and the chlorine dioxide solution (inhibitory substances) during fertility-sensitive period. Then the pollen dyeing rate and the bagged seed-setting rate were calculated and the quantitative real-time PCR was conducted.In addition,the promoters of OsACO5 and OsACS5,which are key enzymes of ethylene synthesis pathway, were isolated and analyzed.[Result]Under 22.5℃ for 7 days, the pollen rate of Zhu 1S and Zhun S was 4.3% and 8.9%, and bagged seed-setting rate was 1.6% and 2.5%, respectively. Compared with the water spray control, the pollen rate of lines Zhu 1S and Zhun S was reduced by 3.3 and 7.2 percent, respectively when spraying with 1600 mg/L ethephon. When spraying with 1500 mg/L of chlorine dioxide solution, the pollen rate was increased by 3.5 and 3.3 percent, and the bagged seed setting rate increased by 0.78 and 0.52 percent, respectively. The expression of OsACO5 and OsACS5 in young panicles was significantly increased at 1600 mg/L of ethephon under 22.5℃ induction for 7 days, which were 1.19, 1.24 (Zhu 1S) and 1.24, 1.1 (Zhun S) times of that of the control (water spray) respectively. When sprayed with 1500 mg/L of chlorine dioxide solution for 7 days,the expression of OsACS5 gene in the Zhu 1S and Zhun S was significantly reduced, which were 0.93 and 0.89 times of that of the control(water spray),respectively,but the expression of OsACO5 was not significantly changed. Compared with normal field temperature (control) for 7 days, water spraying, 1600 mg/L of ethephon and 1500 mg/L of chlorine dioxide solution at 22.5℃ down-regulated the expression of OsACO5 and OsACS5 in young panicles of Zhu 1S and Zhun S. The promoters of OsACO5 and OsACS5 contained multiple tissue-specific expression elements and ethylene-regulated cis-acting elements in addition to the basic structures such as TATA-box and CAAT-box. [Conclusion]Under low temperature condition, spraying 1600 mg/L ethephon in the sensitive period of thermo-sensitive genic male sterile rice had inhibitory effect on fertility conversion from sterility to fertility.关键词
温敏核不育水稻/低温诱导/乙烯利/育性分析Key words
thermo-sensitive genic male sterile rice/low-temperature inducement/ethephon/fertility分类
农业科技引用本文复制引用
李林芝,魏家萍,贾彦凤,刘小林,张海清,王明,麻浩..低温条件下喷施乙烯利对温敏核不育水稻育性的影响[J].中国水稻科学,2018,32(2):128-136,9.基金项目
湖南省财政预算农业专项 （）
国家公益性行业科研专项(201303005). （201303005）
