中国水稻科学2018，Vol.32Issue(2)：189-199,11.DOI:10.16819/j.1001-7216.2018.7107
不同透明度水稻籽粒横断面扫描电镜分析
Scanning Electron Microscopic Analysis of Grain Cross-section from Rice with Different Transparency
摘要
Abstract
[Objective]The objective of this study was to set up an ideal method of observation of a single starch granule cross-section, to clarify the relationship between rice transparency and amylose content and to find the structure foundation that caused the poor rice transparency. [Method] The pre-treated rice grain cross-section and isolated starch were observed under a scanning electron microscopy.[Result]The transparency of both indica and japonica soft rice with amylose content below 15% was significantly decreased. By comparing different methods of grain cross-section pre-treatments, we found that the direct mechanical fracture method can't break a single starch granule and the mechanical fracture with glass scraping method could break down an individual starch granule, however, it will cause rough surface and thus difficult to be analyzed quantitatively. As for the liquid nitrogen fracture method, we found it's an ideal way to break a single starch granule and the cavity in starch granule can be easily observed. We then further analyzed the starch granule arrangement and the internal structure of an individual starch granule in paddy rice from both indica and japonica rice cultivars.We found that all the starch granules arranged tightly and obvious air containing space was observed in an individual starch granule from grains of all dark endosperm rice. Also, we found that the number and area of starch granule cavity in glutinous rice was significantly higher than those in dark endosperm rice. Furthermore, we proved that the number and area of starch granule cavity increased as the grain moisture content decreased.[Conclusion]The direct liquid nitrogen fracture treatment of rice grain cross-section is an ideal method for the single starch granule cross-section observation. Besides, the rice grain transparency showed a close relationship with the moisture content and also the number and size of cavity in the middle of a starch granule but not the starch granule arrangement. Moreover, the size and number of cavities increased as the amylose content decreased.关键词
水稻/淀粉粒/扫描电镜/透明度/气腔Key words
Oryza sativa L./starch/scanning electron microscopy/transparency/cavity分类
农业科技引用本文复制引用
陆彦,张晓敏,祁琰,张昌泉,凌裕平,刘巧泉..不同透明度水稻籽粒横断面扫描电镜分析[J].中国水稻科学,2018,32(2):189-199,11.基金项目
江苏省自然科学基金资助项目(BK20160464) （BK20160464）
江苏省高校自然科学研究面上项目(16KJB210011) （16KJB210011）
国家重点研发计划资助项目(2016YFD0100501) （2016YFD0100501）
国家转基因专项(2016ZX08001006) （2016ZX08001006）
国家自然科学基金资助项目(31561143008 （）
31401354). （）
