中国比较医学杂志2018，Vol.28Issue(3)：52-56,5.DOI:10.3969/j.issn.1671-7856.2018.03.010
瑞舒伐他汀对胶质瘤U87细胞迁移、侵袭的抑制作用
Inhibitory effect of rosuvastatin on migration and invasion of U87 glioma cells in vitro
摘要
Abstract
Objective To investigate the inhibitory effect of rosuvastatin on the migration and invasion of U87 glioma cells and its related mechanisms. Methods Cultured U87 cells were treated with 0, 5, 10 and 20 μmol/L rosuvastatin for 24,48 and 72 h. Cell viability was measured by CCK-8 assay. Migration ability of the cells was detected by scratch assay,and invasion ability of the cells was detected by Transwell assay. The expression of matrix metalloproteinase 2(MMP2),MMP9 and the Wnt/β-catenin signaling pathway-related proteins was detected by Western blot assay. Results Compared with the control group,the viability of U87 cells in the 5,10 and 20 μmol/L rosuvastatin groups was decreased (P < 0.01)in a time- and dose-dependent manner. Migration and invasion abilities of the cells were decreased(P <0.01). In addition,the expression levels of MMP2,MMP9,Wnt1,Wnt3a,Wnt7a,and β-catenin were decreased as well (P < 0.01). Conclusions Rosuvastatin can significantly inhibit the migration and invasion abilities of U87 glioma cells, probably related to the blocking of Wnt/β-catenin signaling pathway.关键词
瑞舒伐他汀/胶质瘤细胞/迁移/侵袭Key words
rosuvastatin/glioma cells/migration/invasion分类
医药卫生引用本文复制引用
刘明,刘熙鹏..瑞舒伐他汀对胶质瘤U87细胞迁移、侵袭的抑制作用[J].中国比较医学杂志,2018,28(3):52-56,5.基金项目
河北省科技支撑计划项目(编号:132777107D). （编号:132777107D）
