中国循证儿科杂志2018，Vol.13Issue(1)：45-49,5.DOI:10.3969/j.issn.1673-5501.2018.01.006
基于16S rDNA可变区测序技术对抗生素治疗后的6月龄内肺炎婴儿的呼吸道菌群现况调查
The respiratory bacteria microbiota of infants with pneumonia less than 6 months old
洪慧慧 1王立波 1祁媛媛1
作者信息
- 1. 复旦大学附属儿科医院 上海,201102
- 折叠
摘要
Abstract
Objective To investigate the composition and characteristics of oropharyngeal and pulmonary bacterial microbiota of infants with pneumonia less than 6 months old.Methods Patients who were diagnosed as pneumonia and aged less than 6 months old were recruited from the respiratory ward of Children's Hospital of Fudan University. The oropharyngeal swabs and bronchoalveolar lavage fluid(BALF)was collected to extract DNA. The V3-V4 region of the bacteria's 16S rDNA gene was amplified by PCR. Sequencing of the purified amplicons was performed and analyzed. Results Overall,24 infants with pneumonia were recruited and further analyzed. The top 5 abundant bacteria in the oropharyngeal were Streptococcus,Pseudomonas,Prevotella 7,Veillonella and Escherichia-Shigella. The top 5 abundant bacteria in the lung were Pseudomonas,Streptococcus,Prevotela 7, Klebsiella and Rothia. The pulmonary microbiota had much more particular bacteria. The oropharyngeal microbiota richness was significantly lower in the 24 patients as compared with the microbiota of the pulmonary(P<0.05). The diversity was significantly lower in pulmonary microbiota(P<0.05). At the genus level, the oropharyngeal microbiota was dominated by Streptococcus(53. 55%)and Pseudomonas(13.39%);the pulmonary microbiota was also dominated by Pseudomonas(73.06%)and Streptococcus (16.91%). The relative abundance of streptococcus and pseudomonas was significantly different between the oropharyngeal and pulmonary microbiota(P<0.05).Conclusion There are significant changes in the oropharyngeal and pulmonary microbiota of infant with pneumonia. The composition of oropharyngeal and pulmonary microbiota is similar and the proportion of each bacteria is different.关键词
婴儿/呼吸道菌群/肺炎Key words
Infant/Pulmonary microbiota/Pneumonia引用本文复制引用
洪慧慧,王立波,祁媛媛..基于16S rDNA可变区测序技术对抗生素治疗后的6月龄内肺炎婴儿的呼吸道菌群现况调查[J].中国循证儿科杂志,2018,13(1):45-49,5.
