中国药科大学学报2018，Vol.49Issue(1)：109-116,8.DOI:10.11665/j.issn.1000 -5048.20180116
硒化肿节风浸膏残渣多糖的制备、工艺优化及抗肿瘤活性研究
Preparative optimization and antitumor activity of selenylaed polysaccharides from the extract residue of Sarcandra glabra（Thunb.）Nakai
摘要
Abstract
Selenylation modification of polysaccharides from the extract residue of Sarcandra glabra(Thunb.) Nakai was operated with sodium selenite as modification agent and barium chloride as the catalyst.The synthesis conditions were optimized by orthogonal experiment with the yields and contents of selenium as indicators.Then, the purity,molecular weight,infrared spectroscopy,thermophysical properties and antitumor activity of the seleny-lated polysaccharides were determined.Results showed that the optimum conditions for preparing selenylated polysaccharides from the extract residue of S.glabra(Thunb.)Nakai were at 70 ℃ for 7 h with 1.0％ nitric acid using 1.5 g barium chloride as catalyst.Under the optimum condition,the physicochemical properties of the derivatives were measured and the results showed that the total carbohydrate content was(94.89 ± 0.83)％,the yield was(21.93 ± 0.85)％,the content of selenium was(944.54 ± 13.91)μg/g,the molecular weight was 1 339 kD. In vitro antitumor activity indicated that exposure of HepG2 cells to the increasing concentrations of the crude polysaccharide from the residue of S.glabra(Thunb.)Nakai and its selenylated products decreased cell viability in dose-dependent and time-dependent manner.The selenium polysaccharide demonstrated a better antitumor activity compared with the raw polysaccharide,which could be explored as a potential antitumor drug from abandoned extracted residue for the functional foods and pharmaceutical industries.关键词
肿节风/浸膏残渣/硒化多糖/制备/抗肿瘤活性Key words
S.glabra(Thunb.)Nakai/extract residue/selenylated polysaccharides/preparation/antitumor activity分类
化学化工引用本文复制引用
胡成,刘雅萌,戴士杰,刘玮,高向东..硒化肿节风浸膏残渣多糖的制备、工艺优化及抗肿瘤活性研究[J].中国药科大学学报,2018,49(1):109-116,8.基金项目
国家自然科学基金资助项目(No.31301473,No.81473216,No.81673589) （No.31301473,No.81473216,No.81673589）
国家重大新药创制重大专项资助项目(No.2012ZX09502001-004) （No.2012ZX09502001-004）
江苏省高校青蓝工程资助项目 （）
江苏省高校优秀科技创新团队资助项目This study was supported by the National Natural Science Foundation of China ( No. 31301473, No. 81473216, No. 81673589) （ No. 31301473, No. 81473216, No. 81673589）
the National Scientific and Technological Major Project for Significant New Drugs Creation ( No. 2012ZX09502001-004) （ No. 2012ZX09502001-004）
the Qing Lan Project of Jiangsu Province （）
and the Project for Outstanding Science and Technology Innovation Team of Universities in Jiangsu Province （）
