中国药理学通报2018，Vol.34Issue(3)：343-347,5.DOI:10.3969/j.issn.1001-1978.2018.03.010
葛根素保护双氧水诱导的SH-SY5Y细胞凋亡
Effects of puerarin on H2O2-induced SH-SY5Y cell apoptosis
摘要
Abstract
Aim To investigate the neuroprotective effects of puerarin on H2O2-induced SH-SY5Y cell ap-optosis and the molecular mechanisms underlying the neuroprotective effects. Methods Neuron injury mod-el was established in vitro through H2O2-induced SH-SY5Y injury. MTT assay was performed to detect the effect of puerarin on H2O2-induced SH-SY5Y survival rates. Hoechst 33342 staining was used to observe the cell apoptosis. JC-1 staining was employed to detect the level of mitochondria membrane poential. Caspase-3 was determined by caspase-3 catalyze the substrate specificity Ac-DEVD-pNA. Caspase-9 was determined by caspase-9 catalyze the substrate specificity Ac-LE-HD-pNA. The effects of puerarin on the protein level of Bcl-2,Bax,p-Akt and Akt were determined by West-ern blot. Results The cell survival rate significantly increased after puerarin pretreatment compared with H2O2model group. Furthermore, puerarin pretreat-ment not only inhibited the decreasing of mitochondrial membrane potential,increasing of caspase-3, caspase-9 enzymatic activity and the expression of Bax,but also promoted the expression of p-Akt and Bcl-2, which was prevented by LY294002, an inhibitor of PI3K/Akt. Conclusion Puerarin can play a neuroprotective role for SH-SY5Y cell apoptosis induced by H2O2, maybe via activating PI3K/Akt signaling pathway.关键词
葛根素/过氧化氢/人神经母细胞瘤/凋亡/PI3K/AktKey words
puerarin/H2O2/SH-SY5Y cells/apop-tosis/PI3K/Akt分类
医药卫生引用本文复制引用
陈龙菊,吴建清,吴太鼎,陈传奇,谭云霞,柯尊记..葛根素保护双氧水诱导的SH-SY5Y细胞凋亡[J].中国药理学通报,2018,34(3):343-347,5.基金项目
国家自然科学基金资助项目(No 81260192) （No 81260192）
湖北省教育厅科学研究重点项目(No D20151903) （No D20151903）
