中国药理学通报2018，Vol.34Issue(3)：358-364,7.DOI:10.3969/j.issn.1001-1978.2018.03.013
毛冬青甲素对大鼠骨髓间充质干细胞增殖及迁移的影响
Effect of ilexonin A on proliferation and migration of bone mesenchymal stem cells in rats
摘要
Abstract
Aim To observe the effect of ilexonin A (IA) on the proliferation of bone marrow mesenchymal stem cells(BMSCs),and to investigate whether IA can promote the migration of BMSCs by up-regulating the expression of CXCR4 in rats. Methods MTT method was used to assay and analyse the proliferation of BM-SCs which were pretreated with different concentrations of IA (3.125,6.25,12.5,25,50,100,200,400, 800 mg·L-1) for 24,48 and 72h,then the best con-centration and the best optimum time were screened. The third generation of BMSCs was exposed to the opti-mal concentration of IA for 48h. The Transwell system was used to carry out the experiment of BMSCs migra-tion. Western blot was used to analyse the expression of CXCR4. Results MTT assay showed that com-pared with control group, the proliferation of BMSCs was significantly reduced in IA 100 ~800 mg·L-1 groups at 24h(P < 0.05); compared with control group, the proliferation of BMSCs significantly de-creased in IA 100~800 mg·L-1groups at 48h(P<0.05),but markedly increased in IA 6.25 and 3.125 mg·L-1groups (P <0.05); compared with control group,the proliferation of BMSCs was significantly re-duced in IA 12.5~800 mg·L-1groups at 72h(P<0.05). The above results indicated that the BMSCs in-cubated with IA 6.25 and 3.125 mg·L-1for 48h were the optimal choice to promote proliferation. The Transwell migration assay showed that incubation with IA 6.25 and 3.125 mg·L-1for 48h could significant-ly increase the migration of BMSCs(P <0.05), and the migration rate was not related with the concentra-tion of IA. This effect was completely blocked by AMD3100(the antagonist of CXCR4). Western blot showed that incubation with IA 6.25 and 3.125 mg· L-1for 48h could increase the expression of CXCR4 in BMSCs(P<0.05). Conclusion IA can promote the proliferation of BMSCs and increase the migration of BMSCs by up-regulating the expression of CXCR4.关键词
骨髓间充质干细胞/迁移/毛冬青甲素/CXCR4/增殖/孵育Key words
bone marrow mesenchymal stem cells/migration/ilexonin A/CXCR4/proliferation/incuba-tion分类
医药卫生引用本文复制引用
李庆双,郑关毅,张碧琴,陈晓东,江琼,刘真臻..毛冬青甲素对大鼠骨髓间充质干细胞增殖及迁移的影响[J].中国药理学通报,2018,34(3):358-364,7.基金项目
福建省自然科学基金资助项目(No 2014J01327) （No 2014J01327）
