中国药理学通报2018，Vol.34Issue(3)：365-370,6.DOI:10.3969/j.issn.1001-1978.2018.03.014
白介素-1受体拮抗剂对大鼠肠缺血/再灌注损伤的保护作用
Protective effect of interleukin-1 receptor antagonist on intestinal ischemia-reperfusion induced injury in rats
摘要
Abstract
Aim To explore the effects of interleukin-1 receptor antagonist (IL-1Ra) on intestinal ischemia-reperfusion (I/R) induced injury in rats. Methods Thirty-five male SD rats were randomly divided into sham operation group (S), model group (I/R), dif-ferent dosage drug groups(C1,C2,C3). Rat intesti-nal I/R model was established via clamping the superi-or mesenteric artery (SMA). After 1 h of ischemia, the arterial clamps were released for 1 h of reperfusion. 10,20,50 mg·kg-1of IL-Ra was injected via caudal vein 15min before reperfusion. Results After 2 h of I/R,compared with S group,I/R group rats exhibited severe damage on the intestinal mucosa, increase in MDA content, decrease in SOD activity, and signifi-cant release of TNF-α, IL-1β, IL-6. The results showed that, following the injection of IL-1Ra after clipping superior mesenteric artery, damage of the in-testinal mucosa was obviously relieved in different dos-age drug groups. Furthermore, there was different de-gree of relief on oxidative stress and inflammatory re-sponses. Conclusion IL-1Ra showed obvious protec-tive effect on intestinal I/R induced injury by relieving oxidative stress and inflammatory response,and it may potentially be used in the clinical treatment of intestinal I/R injury.关键词
白介素-1受体拮抗剂/缺血/再灌注损伤/炎症因子/髓过氧化物酶/丙二醛/超氧化物歧化酶Key words
interleukin-1 receptor antagonist/ische-mic-reperfusion injury/inflammatory factors/my-eloperoxidase/malondialdehyde/muperoxide dis-mutase分类
医药卫生引用本文复制引用
金辰,邹民吉,张冬冬,付文亮,陈瑶,刘青,徐东刚..白介素-1受体拮抗剂对大鼠肠缺血/再灌注损伤的保护作用[J].中国药理学通报,2018,34(3):365-370,6.基金项目
生物安全关键技术研发重点专项(No 2016YFC1202900) （No 2016YFC1202900）
