中国药理学通报2018，Vol.34Issue(3)：388-393,6.DOI:10.3969/j.issn.1001-1978.2018.03.018
莰醇对脂多糖诱导急性肺损伤的保护作用研究
The protective effect of broneol on LPS induced acute lung injury
摘要
Abstract
Aim To investigate the effect of broneol on acute lung injury(ALI) induced by lipopolysaccharide (LPS). Methods Male C57 mice were randomly di-vided into saline group, model group, broneol group and dexamethasone group, then the ALI mouse model was induced by instilling intratracheally with LPS. The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6) and keratinocyte-de-rived cytokine (KC) were measured at 6h, 12h and 24h after instillation of LPS, and the pathological changes of lung were observed. Mice alveolar macro-phages (MHS) and epithelial cells (MLE-12) were stimulated by LPS. After the stimulation of 1h, 3h, 6h,9h, 12h, 24h, the levels of TNF-α and IL-6 in MHS cells and the contents of KC and macrophage in-flammatory protein-2 (MIP-2) in MLE-12 cells were measured. Results Broneol could inhibit the secre-tion of TNF-α,KC and IL-1β;the early effect of bro-neol on IL-6 was not obvious,but the later effect after the treatment of 24 hours was obvious. After LPS instil-lation 6h and 12h,Broneol could significantly improve lung tissue pathological changes. Broneol had no effect on TNF-α secretion of MHS cells, but it obviously af-fected IL-6 secretion in the later stage. In addition, broneol significantly inhibited KC and MIP2 secretion in MLE-12 cells at the later stage of LPS stimulation. Conclusions Broneol can protect LPS-induced acute lung injury. The mechanism may be related to the inhi-bition of the release of inflammatory factors,the activa-tion of inflammatory cells and the aggregation of neutro-phils.关键词
急性肺损伤/莰醇/肺泡巨噬细胞/肺泡上皮细胞/脂多糖/地塞米松Key words
acute lung injury/broneol/alveolar mac-rophages/alveolar epithelial cells/lipopolysaccharide/dexamethasone分类
医药卫生引用本文复制引用
汪雪峰,陈茜茜,崔金瑜,屠思怡,宋顺德,章哲文,汤慧芳..莰醇对脂多糖诱导急性肺损伤的保护作用研究[J].中国药理学通报,2018,34(3):388-393,6.基金项目
国家自然科学基金资助项目(No 81202980) （No 81202980）
浙江省自然科学基金资助项目(No LYY18H310007) （No LYY18H310007）
浙江省医药卫生科技项目资助(No 2018246654) （No 2018246654）
