中国药理学通报2018，Vol.34Issue(3)：412-418,7.DOI:10.3969/j.issn.1001-1978.2018.03.022
马栗种子提取物通过抑制活性氧及JNK途径保护刀豆蛋白A诱导的小鼠急性肝损伤
The hepatoprotective effect of Aesculus hippocastanum seed extract against concanavalin A-induced acute liver injury in mice via inhibition of ROS and JNK pathway
摘要
Abstract
Aim To investigate the effect of Aesculus hippocastanum seed extract(AH) on concanavalin A (ConA)-induced acute liver injury in mice,and to ex-plore whether the mechanism was related to the inhibi-tory effect of AH on oxidative stress and c-Jun N-termi-nal kinase (JNK). Methods ConA(20 mg·kg-1) was administered via tail vein injecting to induce he-patic damage in mice. The groups of AH were given at 12.5,25,50 mg·kg-1by oral gavage separately for 20 days. The serum levels of AST,ALT,TP,and Alb were determined by automatic biochemical analyzer and the A/G ratio was calculated. TNF-α and IFN-γ levels were assayed by ELISA. The liver tissue was attained by HE and the histopathological changes were calculat-ed. The MDA, SOD, GSH contents of liver tissues were assayed by related kits. The activity of caspase-3 was detected by spectrophotometry. The expressions of cytochrome C and Bax, Bcl-2, p-JNK and p-Akt were detected by Western blot. Results The serum levels of ALT, AST, IFN-γ and TNF-α in AH groups were significantly lower than those in ConA-injured group, while the levels of TP,Alb and A/G were significantly higher. The SOD and GSH levels of liver tissues signif-icantly increased and MDA level decreased; liver his-topathological changes were consistent with those of the serological indicators, and AH treatment significantly reduced the pathological damage induced by ConA. In AH group,the expression of cytochrome C,caspase-3, Bax/Bcl-2 ratio and p-JNK markedly decreased, while the expression of p-Akt protein increased compared with ConA model group. Conclusion AH could sig-nificantly protect the ConA-induced acute liver injury in mice via inhibition of ROS and JNK pathway.关键词
马栗种子提取物/刀豆蛋白A/急性肝损伤/氧化应激/c-Jun氨基末端激酶/线粒体凋亡途径Key words
Aesculus hippocastanum seed extract/con-canavalin A/acute liver injury/oxidative stress/c-Jun N-terminal kinase/mitochondrial apoptosis pathway分类
医药卫生引用本文复制引用
吴树金,萨日娜,顾志荣,赵佩,于静,王艳红,葛斌..马栗种子提取物通过抑制活性氧及JNK途径保护刀豆蛋白A诱导的小鼠急性肝损伤[J].中国药理学通报,2018,34(3):412-418,7.基金项目
甘肃省自然科学基金资助项目(No 1606RJZA130) （No 1606RJZA130）
