中国药理学通报2018，Vol.34Issue(3)：418-422,5.DOI:10.3969/j.issn.1001-1978.2018.03.023
基于NOD2/RIP2/NF-κB信号通路研究柚皮素对新生大鼠缺血缺氧性脑损伤的保护作用
Protective effects of naringenin against hypoxic-ischemic brain damage in neonatal rats through suppression of NOD2/RIP2/NF-κB pathway
摘要
Abstract
Aim To explore the protective effects of naringin on hypoxic ischemic brain injury in neonatal rats and its mechanism. Methods Ninety-six healthy 7-day neonatal SD rats were randomly divided into sham operation group, hypoxic-ischemic brain damage group (HIBD group),HIBD with low-dose naringenin group(50 mg·kg-1, NG-L) and HIBD with high-dose naringenin group(100 mg·kg-1,NG-H). Neu-rological deficit, HE staining and brain water content were measured 48h after operation. Immunoblotting was used to detect the expressions of NOD2,RIP2 and NF-κB. Enzyme linked immunosorbent assay(ELISA) method was adopted to detect TNF-α and IL-1β ex-pression. Results Compared with HIBD group, the neurological deficit score decreased, the pathological damage was reduced, and the water content of brain tissues markedly decreased by naringenin(50,100 mg ·kg-1) treatment(P<0.05). Western blot revealed the down-regulation of NOD2,RIP2 and NF-κB by na-ringenin (50,100mg·kg-1) treatment (P<0.05). The content of TNF-α and IL-1β in brain tissues was lower than that of HIBD group (P <0.05). Conclu-sion Naringenin is likely to exert a protective role in neonatal rats of hypoxic ischemic brain injury perhaps through decreasing the expression of NOD2, RIP2 and NF-κB,and reducing the secretion of TNF-α and IL-1β.关键词
柚皮素/缺氧缺血性脑损伤/炎症/NOD2/RIP2/NF-κBKey words
naringenin/hypoxic-ischemic brain dam-age/inflammation/NOD2/RIP2/NF-κB分类
医药卫生引用本文复制引用
冯祝婷,冯占辉,王世平,靳艳玲,肖美,叶兰..基于NOD2/RIP2/NF-κB信号通路研究柚皮素对新生大鼠缺血缺氧性脑损伤的保护作用[J].中国药理学通报,2018,34(3):418-422,5.基金项目
贵州省科技厅基金(黔科合J字[2012]2061) （黔科合J字[2012]2061）
贵州省科技计划项目[黔科合平台人才 (2016)5625] （2016）
