中国药理学通报2018，Vol.34Issue(3)：428-432,5.DOI:10.3969/j.issn.1001-1978.2018.03.025
加味脑泰方对去势脑缺血大鼠脑损伤的影响与雌激素水平相关性研究
Effect of Jiawei Naotaifang on cerebral infarction area and level of estrogen of ovariectomized rats with cerebral ischemia and its correlation
摘要
Abstract
Aim To investigate the effects of Jiawei Naotaifang on cerebral infarction area, pathological changes of brain tissue and estrogen level of focal cere-bral Iischemia in female ovariectomized rats, and cor-relation between estrogen levels and cerebral infarction area. Methods SD rats were randomly divided into sham operation group, ovariectomized group, cerebral ischemia group,model group,and drug groups(estro-gen group, Jiawei Naotaifang high dose group, Jiawei Naotaifang middle dose group, Jiawei Naotaifang low dose group). The rats in the ovariectomized group, model group, drug groups were ovariectomized, elev-enth days after the ovariectomy. The rats in the drug groups were given intragastric administration for three days. The rats in the model group, cerebral ischemia group and drug groups were prepared for cerebral is-chemia models. Neurological function scores were scored 24 hours after the success of the model, serum levels of estrogen were detected, and the brain was stained with 2, 3, 5-triphenyltetrazolium chloride (TTC) and hematoxylin-eosin staining(HE), TTC staining was used to measure the area of cerebral in-farction, and HE staining was used to observe the pathological changes of brain tissues. Results Com-pared with cerebral ischemia group,cerebral infarction area of rats in the model group increased significantly, the estrogen level was lower and the necrosis and py-knosis of cortical and hippocampus cells of rats in the model group were more obvious. Compared with model group,the cerebral infarction area of rats in the drug groups was reduced,the estrogen levels were elevated, especially in Jiawei Naotaifang high dose group and es-trogen group. The cell morphology of rats,in the estro-gen group,Jiawei Naotaifang high dose group and mid-dle dose group, was improved obviously. Cerebral in-farction area was negatively correlated with the level of estrogen. Conclusions The cerebral infarction area of cerebral ischemia in female ovariectomized rat is signif-icantly correlated with the level of estrogen. Jiawei Naotaifang can reduce the damage and alleviate brain injury of cerebral ischemia in female ovariectomized rats,which may be related to the improvement of estro-gen level.关键词
加味脑泰方/脑缺血/脑梗死面积/雌激素/细胞形态/相关性Key words
Jiawei Naotaifang/cerebral ischemia/in-farct size/estrogen/cell morphology/correlation分类
医药卫生引用本文复制引用
秦莉花,葛金文,刘洋,黄娟,成邵武,刘林,李晟,王国佐,龚胜强,程成..加味脑泰方对去势脑缺血大鼠脑损伤的影响与雌激素水平相关性研究[J].中国药理学通报,2018,34(3):428-432,5.基金项目
湖南省教育厅优秀青年科研项目(No 17B196) （No 17B196）
湖南省教育厅一般科研项目(No 14C0857) （No 14C0857）
湖南省中医药管理局课题(No 201784) （No 201784）
