中国药业2018，Vol.27Issue(5)：1-3,3.DOI:10.3969/j.issn.1006-4931.2018.05.001
丹参川芎嗪对兔耳增生性瘢痕的抑制作用研究
Inhibitory Effect of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection on Hypertrophic Scars of Rabbit Ears
摘要
Abstract
Objective To observe the inhibitory effect of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection on hypertrophic scars of rabbit ears. Methods Totally 16 healthy Japanese white big-ear rabbits were selected to establish animal model of hyper-trophic scars of rabbit ears. After the wound epithelialization ( 21 d after injury ) , the rabbits were randomly divided into 4 groups, 4 rabbits in each group. The control group ( group A ) was partially injected with normal saline, and the experimental groups ( group B, C, D ) were partially injected with low, medium and high concentration of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection. Scar thickness was measured on the 32nd and 56th day after injury, and HE staining was routinely performed, the content of HPr in scar tissue was determined by chloramine-T method. The expression and changes of proliferating cell nuclear antigen ( PCNA ) in scar tissue were observed by immunohistochemistry. Results In the experimental group, the thickness of scar, the content of HPr and the positive expression rate of PCNA were negatively correlated with the drug concentration ( P < 0. 05 ) . The collagen content and PCNA protein level in the scar tissue of the experimental group were significantly decreased, showing a dose-response relationship, and the difference was statistically significant compared with the control group ( P < 0. 01 ) . In the experimental group, there was significantdifferenceamong the subgroups on the 32nd and 56th days after injury ( P < 0. 01 ) . Conclusion Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection can inhibit the proliferation of hypertrophic scar tissue in rabbit ears by reducing the level of collagen synthesis and the expression of PCNA protein, which can provide new options for the treatment of hypertrophic scars.关键词
丹参川芎嗪注射液/增生性瘢痕/增殖细胞核抗原/兔耳Key words
Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection/hypertrophic scar/proliferating cell nuclear antigen/rabbit ears分类
医药卫生引用本文复制引用
冯登超,高栋梁,白翠翠,高东东,武斌,薛宏斌,杨喜明..丹参川芎嗪对兔耳增生性瘢痕的抑制作用研究[J].中国药业,2018,27(5):1-3,3.基金项目
陕西省自然科学基础研究计划项目[ 2014JM4199 ]. （）
