中国药业2018，Vol.27Issue(5)：10-12,3.DOI:10.3969/j.issn.1006-4931.2018.05.004
β-环糊精修饰毛细管区带电泳测定三黄片中大黄有效成分含量
Content Determination of Active Ingredient of Rheum Palmatum in Sanhuang Tablets by β-Cyclodextrin Modified Capillary Electrophoresis
摘要
Abstract
Objective To establish a β-cyclodextrin modified capillary electrophoresis method for content determination of emodin and chrysophanol in Sanhuang Tablets. Methods Active ingredient of Rheum palmatum was acquired with ultrasonic-assisted extraction. The detection was carried out with an uncoated fused-silica capillary ( 75 μm × 67. 4 cm, the effective length was 51 cm ) . The buffer was consisted of 60 mmol/L Na2B4O7+40 mmol/L Na2CO3, and buffer additives was 40 mmol/L β-cyclodextrin ( pH=8. 9 ) . The detection wavelength was 254 nm. Results RSDs of repeatability test of emodin and chrysophanol were 1. 76％ and 1. 53％ ( n=6 ) . The linear ranges of active ingredient were 2. 0-18. 5 μg/mL and 2. 50-1. 56 × 103 μg/mL. The average recoveries were 93. 53％ and 97. 26％, and RSD were 0. 83％ and 2. 52％ ( n=3 ) . Conclusion The method is simple, accurate and reproducible, which can be used for content determination of active ingredient of Rheum palmatum in Sanhuang Tablets.关键词
三黄片/高效毛细管电泳法/大黄素/大黄酚Key words
Sanhuang Tablets/high performance capillary electrophoresis/emodin/chrysophanol分类
医药卫生引用本文复制引用
曾雪,陈竹,刘应杰,杨元娟,夏培元..β-环糊精修饰毛细管区带电泳测定三黄片中大黄有效成分含量[J].中国药业,2018,27(5):10-12,3.基金项目
重庆市科委基础与前沿项目[ cstc2014jcyjA10125 ] （）
重庆市卫计委中医项目[ zy20150242 ] （）
重庆市卫计委医学项目[ 2012-1-093 ] （）
重庆市教育委员会新技术推广项目 [ GZTG201604 ] （）
重庆医药高等专科学校科学技术研究项目[ ygz2015101 ]. （）
