中国药业2018，Vol.27Issue(5)：13-15,3.DOI:10.3969/j.issn.1006-4931.2018.05.005
高效液相色谱法同时测定六味补血胶囊中没食子酸、芍药苷和藁本内酯含量
Simultaneous Determination of Gallic Acid, Paeoniflorin and Ligustilide in Liuwei Buxue Capsules by HPLC
摘要
Abstract
Objective To establish an HPLC method for content determination of gallic acid, paeoniflorin and ligustilide in Liuwei Buxue Capsules. Methods The Symmetry Shield RP18 ODS column ( 250 mm × 4. 6 mm, 5 μm ) was adopted with gradient elution, the mobile phase was acetonitrile-0. 1％ phosphoric acid aqueous solution, the flow rate was 1. 0 mL/min, the mode of wavelength switching was used, the detection wavelength was 280 nm for gallic acid, 230 nm for paeoniflorin and 334 nm for ligustilide. Results The linear ranges of gallic acid, paeoniflorin and ligustilide were 0. 046-0. 910 g/L, 0. 049-0. 970 g/L, 2. 1-52. 3 μg/mL. The average recoveries were 99. 20％, 98. 79％, 97. 15％. RSDs were 0. 29％, 0. 97％, 0. 81％ ( n=6 ) . Conclusion The method is accurate, reliable and reproducible, which can be used for the quality control of Liuwei Buxue Capsules.关键词
六味补血胶囊/没食子酸/芍药苷/藁本内酯/高效液相色谱法/含量测定Key words
Liuwei Buxue Capsule/gallic acid/paeoniflorin/ligustilide/HPLC/content determination分类
医药卫生引用本文复制引用
关潇滢,曾利娜,胡晓谕,温晓丽,赵渝,肖丽和..高效液相色谱法同时测定六味补血胶囊中没食子酸、芍药苷和藁本内酯含量[J].中国药业,2018,27(5):13-15,3.基金项目
广东省深圳市科技计划项目[ JCYJ20140722104416530 ]. （）
