摘要
Abstract
Objective To investigate the dynamic drift correction coefficient for detection of valproic acid concentration by enzyme multi-plied immunoassay technique ( EMIT ) . Methods 50 μg/mL valproic acid standard water solution was taken as the quality control sample. The automatic biochemical analyzer made in Germany Siemens was used to do the quality control test at the 48th,108th tests of a kit, in order to speculate the dynamic drift exponential function of the quality control detection concentration. Results The dynamic drift coefficient was obtained: k=( 1+0. 00066 × n0. 55535 ) n, and the dynamic correction coefficient was obtained: k′=1/ [ ( 1+0. 00066 × n0. 55535 ) n ] . The detection concentration of the 1st, 20th, 31st, 97th 120th and 135th quality control samples were used to verify the calculation, and the relative errors were between -6％ and 6％. Conclusion The obtained dynamic drift correction coefficient can be used for the dynamic correction of quality control concentration of valproic acid, which is conducive to the accurate detection of blood concentration of valproic acid, and it can reduce the waste of reagents produced by the irregular calibration or quality control of the valproic acid detection system.关键词
酶放大免疫测定技术/丙戊酸/动态飘逸/校正系数/试剂瓶底效应Key words
enzyme multiplied immunoassay technique/valproic acid/dynamic drift/correction coefficient/reagents bottle base effect分类
医药卫生
