神经药理学报2017，Vol.7Issue(2)：42,1.
The Effects of Dendrobium Nobile Lindl.Alkaloids(DNLA)on α,β and γ-secretase of Hippocampal Neurons in SD Rat
The Effects of Dendrobium Nobile Lindl. Alkaloids (DNLA)on α,β and γ-secretase of Hippocampal Neurons in SD Rat
摘要
Abstract
Objective:Alzheimer's disease(AD)is a chronic neurodegenerative disorder characterized by the neuropathological conclusion of intracellular neurofibrillary tangles and extracellular amyloid plaque accumulation in vulnerable regions of the brain. The accumulation of amyloid-β peptides(Aβ)plays a key role in AD pathogenesis. The molecular culprit in AD is the Aβ,which derives from the amyloid precursor protein(APP)through sequential cleavage by the two proteasesβ- andγ-secretase. In contrast,α-secretase cleaves APP within the Aβdomain,thus preventing Aβgeneration. In this study,we tried to investigate the effects of Dendrobium nobile Lindl. Alkaloids (DNLA)on α,βandγ-secretase of hippocampal neurons in SD Rat. Methods:Primary hippocampal neurons of rat were cultured till the 8th day. Neurons purity was detected by immunofluorescence technique. The neurons were exposed to DNLA(0.35,3.5,35,350,700 ng·mL-1,respectively), then cultured for another 48 h and used for further detection. The cells activity was measured by methylthiazoldiphenyl tetrazolium(MTT)assay. Western blot measured the protein expression of APP,α-secretase(ADAM10 and ADAM17),β-secretase(BACE1),γ-secretase(PS1)and Aβ42. Results:Neurons purity was,at least,84.5％. The cell activity of viable hippocampal neurons was not changed significantly after the treatment with DNLA. However,DNLA reduced the protein expression of APP, ADAM10,BACE1 and Aβ42 of hippocampal neurons,increased the protein expression of ADAM17 and PS1,but the PS1 not significantly. Conclusion:DNLA reduced the protein expression of APP, ADAM10,BACE1 of hippocampal neurons in rat,increased the protein expression of ADAM17. And then reduced the protein expression of Aβ42. It suggests that DNLA has a potential health care value.关键词
Alzheimer's disease/Dendrobium nobile Lindl. Alkaloids/hippocampal neurons/α-secretase/β-secretase/γ-secretase/Aβ42Key words
Alzheimer's disease/Dendrobium nobile Lindl. Alkaloids/hippocampal neurons/α-secretase/β-secretase/γ-secretase/Aβ42引用本文复制引用
HUANG Juan,XU Yun-yan,ZHANG Ming-hui,YANG Xiao-hui,LIU Yuan,WU Qin,SHI Jing-shan..The Effects of Dendrobium Nobile Lindl.Alkaloids(DNLA)on α,β and γ-secretase of Hippocampal Neurons in SD Rat[J].神经药理学报,2017,7(2):42,1.基金项目
The work described in this paper was supported by the NSFC(NO.81473201,81660685)and Science Research Project of Education Department in Guizhou Province(NO. KY[2015]373). （NO.81473201,81660685）
