浙江医学2018，Vol.40Issue(1)：16-18,46,4.DOI:10.12056/j.issn.1006-2785.2017.40.1.2017-543
IL-6/sIL-6R复合物对血管内皮细胞VEGF及MMP-9表达的影响
IL-6/sIL-6R complex up-regulates VEGF and MMP-9 expression in human umbilical vein endothelial cells
蔺娜 1宋欣伟 1张缪佳2
作者信息
- 1. 310006杭州,浙江省中医院风湿科
- 2. 南京医科大学附属第一医院风湿科
- 折叠
摘要
Abstract
Objective To investigate the regulatory effect of IL-6/slL-6R complex on expression of MMP-9 and VEGF in human umbilical vein endothelial cells (HUVECs).Methods Cultured HUVECs were treated with slL-6R (100ng/ml) and various concentrations of IL-6 (0,10,20,40 and 80ng/ml) for 24h,the expression of MMP-9 and VEGF was detected by real-time fluorescent quantitative PCR and Western blot.Results The mRNA and protein expressions of MMP-9 and VEGF were increased in presence of IL-6/sIL-6R complex (P＜0.05).Conclusion IL-6/sIL-6R complex can promote the expression of MMP-9 and VEGF in human umbilical vein endothelial cells,indicating that IL-6/sIL-6R complex may be involved in the pathogenesis of rheumatoid arthritis.关键词
白介素-6/可溶性白介素-6受体/类风湿关节炎/基质金属蛋白酶-9/血管内皮生长因子Key words
IL-6/sIL-6R/Rheumatoid arthritis/MMP-9/VEGF引用本文复制引用
蔺娜,宋欣伟,张缪佳..IL-6/sIL-6R复合物对血管内皮细胞VEGF及MMP-9表达的影响[J].浙江医学,2018,40(1):16-18,46,4.
