朱杭 1林士明 1王栋 1诸力 1何永江 1王坚 1潘浩1
作者信息
- 1. 310007杭州,浙江中医药大学附属广兴医院骨科
- 折叠
摘要
Abstract
Objective To determine the effect of NF-κB and ASIC3 in nerve root pain induced by exposure to nucleus pulposus in rats and the related mechanisms.Methods SD rats were randomly divided into control group (sham,n=18),inflammatory group(NP,n=16),inflammatory+amiloride group(Ami,n=16),inflammatory+PDTC group (PDTC,n=16),inflammatory +amiloride+PDTC group (Ami+PDTC,n=16).The nerve root inflammation model was induced by exposure of L5 dorsal root ganglion (Ls DRG) to rat nucleus pulposus (NP).NF-κB signaling pathway blocker PDTC (20μg/kg) or/and ASIC3 signaling pathway blocker amiloride (20μg/kg) were injected intraperitonealy to rats in PDTC group,Ami group and Ami+PDTC group,respectively.The mechanical pain withdrawal threshold (PWT) was detected at 2,4,6,8,10 and 12 days after modeling,respectively.The expression of ASIC3,NF-κB p65,TNF-α,IL-6 mRNA and protein in DRG were examined by real-time PCR and Western blot,respectively.TNF-α and IL-6 concentrations in peripheral blood and DRG were determined at 1,3,7 and14 days after the surgical procedures with ELISA method,and NO levels were examined with biochemistry test.Results Rats exposed to the NP showed decreased PWT for 12 days (P＜0.01),and the expression of ASIC3,NF-κB and the levels of NO,TNF-α IL-6 were up-regulated in DRG induced by NP 14 days after surgery (all P＜0.01).After administration of amiloride and PDTC,the mechanical PWT decreased for 8 days after surgery and then gradually increased in amiloride groups and/or PDTC groups.Furthermore,a significant increase in PWT in amiloride and/or PDTC groups compared with the NP model group were observed at day 10 and day 12 after surgery(all P＜0.01).The expression of ASIC3 and NF-κB in DRG was down-regulated(both P＜0.05),and the serum levels of NO,TNF-α,IL-6 were significantly decreased for 14 days (all P＜0.01).Conclusion The interaction of NF-κB and ASIC3 may alleviate nerve root pain induced by exposure to NP through inhibition of NO and inflammatory cytokines TNF-α and IL-6 levels.关键词
椎间盘/髓核/核因子-κB/ASIC3/炎症Key words
Intervertebral disc/Nucleus pulposus/NF-κB/ASIC3/Inflammation
