浙江医学2018，Vol.40Issue(2)：121-125,5.DOI:10.12056/j.issn.1006-2785.2018.40.2.2017-920
细梗香草皂苷联合吉西他滨对胰腺癌细胞BxPC-3抑制作用的研究
Effect of lysimachia capillipes with gemcitabine on proliferation and apoptosis of pancreatic cancer BxPC-3 cells and related mechanism
摘要
Abstract
Objective To investigate the effect of lysimachia capillipes(LC) and gemcitabine(GEM) on proliferation and apoptosis of human pancreatic cancer cell line BxPC-3 in vitro.Methods The cultured BxPC-3 cells were treated with GEM (1μmol/L,group B),LC (15μg/ml,group C) and GEM with LC (15μg/ml and 1μmol/L,group D) for 48h,respectively.The untreated BxPC-3 cells served as controls (group A).The proliferation of BxPC-3 cells was measured by MTS method,half maximal inhibitory concentration(IC50) and combined index(CI) of the two drugs were analyzed with Calcusyn software.Cell apoptosis was examined by using flow cytometry(FCM) with Annexin V/PI staining solution.The expression of proteins PARP and Capase-3 was detected by Western blot.Results The proliferation of BxPC-3 cells was inhibited by LC and GEM.The IC50 value of LC and GEM were 16.55μg/ml and 1.27μmol/L,the CI value was 0.53-0.65,when BxPC-3 cells treated with 15-20μg/ml LC and 1-30μmol/L GEM.The apoptosis rate of BxPC-3 cells was increased in group B,C and D compared with group A (all P＜0.01),and the effect of group D is stronger than that of group B and C(both P＜0.01).The expression of Capase-3 protein was up-regulated in group B,C and D.Compared with A (all P＜0.05),and the expression of PARP protein was down-regulated in group C and D Cormpared with A(both P＜0.05).Conclusion LC and GEM can inhibit the proliferation of BxPC-3 cells,and the effect of combination of the two drugs is more marked.LC can induce apoptosis of BxPC-3 cells by activating the Caspase-3 protein expression and decomposing PARP protein.关键词
胰腺癌/细梗香草皂苷/吉西他滨/PARP/Caspase-3/细胞凋亡Key words
Pancreatic cancer/Lysimachia capillipes/Gemcitabine/PARP/Capase-3/Cell apoptosis引用本文复制引用
于贤金,何亚红,张筱凤..细梗香草皂苷联合吉西他滨对胰腺癌细胞BxPC-3抑制作用的研究[J].浙江医学,2018,40(2):121-125,5.基金项目
浙江省中医药科技计划项目(2013ZZ012) （2013ZZ012）
