浙江医学2018，Vol.40Issue(2)：126-129,后插1-后插2,6.DOI:10.12056/j.issn.1006-2785.2018.40.2.2017-2001
飞秒激光角膜基质透镜用于周边板层角膜移直的实验研究
Application of corneal stromal lenticules in heterogeneous peripheral lamellar keratoplasty in rabbits
摘要
Abstract
Objective To evaluate the application of cryopreserved lenticules extracted from small incision lenticule extraction (SMILE) surgery in heterogeneous peripheral lamellar keratoplasty in of rabbits with peripheral corneal stromal defect.Methods Ten New Zealand white rabbits were randomly divided into 2 groups.Peripheral corneal stromal defect model was constructed in right eye of each rabbit.Two layers of cryopreserved lenticules were stacked up for use.In group A,the two-layer graft was sewed to the right eyes.In group B,fibrin glue was coated between the two layers to prepare the graft,and the graft and plant bed were adhered with glue before sewing.The smoothness of graft,corneal reepithelialization status,peripheral corneal condition,the graft-host fitness,and the transparency of the graft were observed.Histopathological examinations were conducted in the end of observation.Results Both groups had complete reepithelialization,smooth graft surface and good graft-host fitness were observed 3-5 days after operations.Four grafts in group A and 1 graft in group B were completely transparent in 1 month,and histopathological examination showed normal corneal stromal cells in these eyes.While 1 graft in group A and 4 grafts in group B had extreme immuno-inflammatory response and enucleation was performed in these eyes.Histopathological examinations showed severe edema,opacity and grey suppurative infiltration.Conclusion The study demonstrates that the application of cryopreserved lenticules extracted from SMILE surgery appears to be feasible and effective in heterogeneous peripheral lamellar keratoplasty in rabbits with peripheral corneal stromal defect.关键词
飞秒激光/角膜基质透镜/周边板层角膜移植/纤维蛋白胶Key words
Femtosecond laser/Corneal stromal lenticules/Peripheral lamellar keratoplasty/Fibrin glue引用本文复制引用
高颖,张俐娜,何娜,杨咏,厉青青,程雪莹..飞秒激光角膜基质透镜用于周边板层角膜移直的实验研究[J].浙江医学,2018,40(2):126-129,后插1-后插2,6.基金项目
浙江省科技计划项目(2015C33282) （2015C33282）
