浙江医学2018，Vol.40Issue(2)：134-137,141,5.DOI:10.12056/j.issn.1006-2785.2018.40.2.2017-1013
胃液长链非编码RNA RMRP的检测及其临床价值分析
Detection of long non-coding RNA RMRP expression levels in gastric juice and its clinical significance
摘要
Abstract
Objective To investigate the expression of long non-coding RNA (IncRNA) component of mitochondrial RNA processing endoribonuclease(RMRP) in gastric juice and its clinical significance.Methods The expression levels of RMRP were detected by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) in gastric juice samples from 45 cases of normal mucosa or minimal gastritis (NMMG),30 cases of gastric ulcer (GU),16 cases of atrophic gastritis (AG) and 39 cases of gastric cancer (GC).The correlation between gastric juice RMRP level and gastric cancer was analyzed.Using receiver operating characteristic (ROC)curve the sensitivity and specificity of RMRP in diagnosis of gastric cancer were evaluated,and compared with serum CEA and gastric juice CEA for diagnosis of early GC and advanced GC.Results There was no significant difference in expression of gastric juice RMRP between NMMG group and GU group (P ＞0.05),while gastric juice RMRP levels were significantly decreased in AG group and increased in GC group(both P＜0.01).The expression levels of RMRP in GC group were significantly associated with age and H.pylori infection of patients (both P＜0.05).The sensitivity and specificity of RMRP in gastric juice for diagnosis of GC were 0.56 and 0.75,respectively.Combined gastric juice RMRP with CEA in serum and gastric juice improved the detection rate of GC compared to serum CEA alone(all P＜0.05).Conclusion RMRP in gastric juice may be used as a potential screening marker for gastric cancer.关键词
胃癌/长链非编码RNA/RMRP/临床意义Key words
Gastric cancer/LncRNA/RMRP/Clinical significance引用本文复制引用
周善学,朱春霞,陆蓉丹,邵永富,叶国良..胃液长链非编码RNA RMRP的检测及其临床价值分析[J].浙江医学,2018,40(2):134-137,141,5.基金项目
浙江省医药卫生科技计划项目(2017KY598) （2017KY598）
