浙江医学2018，Vol.40Issue(2)：142-144,3.DOI:10.12056/j.issn.1006-2785.2018.40.2.2017-1966
PM2.5氧化应激对变应性哮喘气道上皮固有免疫调控因子表达的刺激作用
Stimulatory effect of PM2.5 oxidative stress on expression of innate immune regulatory factors in airway epithelia of allergic asthma mice
摘要
Abstract
Objective To investigate the stimulatory effect of PM2.5 oxidative stress on the expression of innate immune regulatory factors in airway epithelia of allergic asthma mice.Methods Twenty four BALB/c female mice were randomly divided into 4 groups with 6 in each group:normal control group,asthma model group,PM2.5 mild pollution group and PM2.5 severe pollution group.The asthma model was induced by intraperitoneal injection of ovalbumin (OVA).Mice in asthma model group,PM2.5 mild pollution group and PM2.5 severe pollution group were stimulated by inhalation of 1％ OVA solution,PM2.5 90μg/m3+1％OVA and PM2.5 200μg/m3+1％OVA,respectively.Serum levels of IL-4,IL-5,IL-13 and IFN-γ were detected,and the pathological changes of airway and lung tissues were observed in all groups.Results Serum levels of IL-4,IL-5,IL-13 and IFN-γ in PM2.5 pollution groups were significantly higher than those in asthma model group (all P＜0.05);and the levels in PM2.5 severe pollution group were higher than those in PM2.5 mild pollution group (all P＜0.05).The pathological examination showed that the changes in lung structure were increased,airway epithelial thickening aggravated,vascular infiltration of inflammatory cells and lumen stenosis or occlusion were more severe with the increased PM2.5 pollution.Conclusion PM2.5 may increase the risk of allergic asthma,which may be associated with the increased secretion of immune regulatory factors in the airways;and the higher the PM2.5,the more pronounced the effect.关键词
PM2.5/氧化应激/变应性哮喘/免疫Key words
PM2.5/Oxidative stress/Allergic asthma/Immunity引用本文复制引用
鲍志坚,胡旭,沈晓强..PM2.5氧化应激对变应性哮喘气道上皮固有免疫调控因子表达的刺激作用[J].浙江医学,2018,40(2):142-144,3.基金项目
浙江省医药卫生科技计划项目(2015KYB312) （2015KYB312）
