中山大学学报（医学科学版）2018，Vol.39Issue(2)：178-185,226,9.
肝癌患者肝移植前外周血免疫表型分析
Detection of Immune Cell Subsets of Peripheral Blood in Hepatocellular Carcinoma before Liver Transplantation
摘要
Abstract
[Objective]To investigate the characteristics of immunophenotypes in hepatocellular carcinoma(HCC) before liver transplantation.[Methods]The immunophenotypes of T-,B- cells,monocytes,dendritic cells(DC)and NK-cells in peripheral blood from 6 HCC patient who were ready to have liver transplantation and 6 healthy volunteers were analyzed by multicolor flow cytometry.[Results]In the patients,the proportions of CD4+PD-1+T cells,Treg cell (CD4+CD25+CD39+T cells),CD19+B cells,Plasmablasts(CD27highCD38highIgD-IgM-),classical monocytes(CD14high CD16-)and mature NK-cells(CD3-CD56high)were all higher than those in the healthy controls(all P<0.05).However, marginal zone B cell(CD27+IgD+),Non-switched B cells(CD27+CD38dimIgM+),intermediate monocytes(CD14high CD16+)and immature NK-cells(CD3-CD56+)were lower than those in the healthy controls(all P<0.05). And there wasn't any obvious difference in quantity being observed among other cell types.[Conclusion]There was difference in the immunophenotypes of immune cells in peripheral blood between HCC patients before liver transplantation and healthy people.And this finding exerts important effects on monitoring the immune status of the patients after liver transplantation and guiding the administrations of immunosuppressors.关键词
肝癌/肝移植/免疫细胞亚群/多色流式细胞术Key words
liver cancer/liver transplantation/immunophenotyping/multi-color flow cytometry分类
医药卫生引用本文复制引用
邓小红,张倩倩,蔡燕,欧阳涓,陈茂根,唐秀鑫,何晓顺,黄俊琪..肝癌患者肝移植前外周血免疫表型分析[J].中山大学学报（医学科学版）,2018,39(2):178-185,226,9.基金项目
国家自然科学基金(31370870) （31370870）
广东省自然科学基金(S2013020013000) （S2013020013000）
广东省科技计划项目(2013A020229003) （2013A020229003）
广州市科技计划项目(201604020083) （201604020083）
广东省器官捐献与移植免疫重点实验室建设项目(2013A061401007) （2013A061401007）
广东省器官移植国际合作基地建设项目(2015B050501002) （2015B050501002）
