中山大学学报（医学科学版）2018，Vol.39Issue(2)：227-232,6.
Expressmarker22系统检测广东汉族人群中的标准梯度外等位基因
Off-ladder Alleles Detected by Expressmarker22 Kit in Guangdong Han Population
摘要
Abstract
[Objective]To study the rare alleles frequencies and sequences of Expressmarker 22 kit in Guangdong Han Population.[Methods]3495 Samples from unrelated individuals in Guangdong Han Population were screened by using AGCU Expressmarker 22 kit(EX22)and ABI 3100 Genetic Analyzer. Then analyzed the frequencies of the off-ladder(OL)alleles and sequenced the rare alleles obtained based on comparison with the STRBase database and litera-ture.[Results]33 off-ladder alleles with 25 rare alleles were found in 10 STR loci,and allele frequencies ranged from 0.0003~0.0046. Sequencing of the11unreported rare OL alleles showed that most of them have incomplete repeats.[Conclusion]Off-ladder alleles especially the rare alleles are helpful to improve the power of discrimination and the power of exclusion and to provide samples which will be added its allele into ladders These OL-alleles will supplement forensic DNA database.关键词
Expressmarker22/短串联重复序列/梯度标准物范围之外的等位基因/稀有等位基因/测序Key words
EX22/short tandem repeat/off-ladder allele/rare allele/sequence分类
医药卫生引用本文复制引用
马晓燕,李少英,何文智,王燕超,王晓蔓,冼嘉嘉,黎青..Expressmarker22系统检测广东汉族人群中的标准梯度外等位基因[J].中山大学学报（医学科学版）,2018,39(2):227-232,6.基金项目
广东省科技计划项目(2013B022000023) （2013B022000023）
