中国现代中药2018，Vol.20Issue(2)：152-158,7.DOI:10.13313/j.issn.1673-4890.20170605003
基于人工胃酸水解的黄芪糖指纹图谱的建立及不同种质资源黄芪鉴别
Establishment of Fingerprint of Astragali Radix Polysaccharides Based on Artificial Acidic Acid Hydrolysis and Identification of Different Germplasm Resources of Astragali Radix
摘要
Abstract
Objective:The simulated artificial acid solution was used to treat soluble polysaccharides and glycocon-jugates of Astragalus cells in different germplasm resources of cultivated and wild Astragalus membranaceus var.mongholicus and A.membranaceus,and a series of PACE saccharide fingerprints for the identification and evaluation of the germplasm resources of Astragali Radix.Methods:The data were analyzed by principal component analysis and t-test using SMICA software to ob-tain the difference between different germplasm resources Astragali Radix.Results:The cultivated A.membranaceus var.mongholicus and wild A.membranaceus var.mongholicus separated significantly,tetrasaccharide and hexasaccharide in the polysaccharides acid hydrolyzate could be used as the main differentially expressed sugar segment to distinguish the growth pat-tern.The content of the tetrasaccharide in wild A.membranaceus was higher than that A.membranaceus var.mongholicus,and the content of hexose in the cultivated A.membranaceus var.mongholicus washigher than that of the wild A.membranaceus var.mongholicus.Trisaccharide and tetrasaccharide could be used as the main difference between wild A.membranaceus var.mongholicus and wild A.membranaceus.The pentasaccharide and hexasaccharidecould be used as the main differentially expressed fragments to distinguish the cultivated A.membranaceus var.mongholicus and A.membranaceus.Conclusion:Artifi-cial acid-degrading polysaccharide product can well distinguish Astraglaus species and growth mode.Thie study laid the founda-tion for the quality evaluation of Astragali Radix.关键词
人工胃酸/黄芪/生长方式/多糖/指纹图谱Key words
Artificial acid/Astragali Radix/growth mode/polysaccharide/fingerprint引用本文复制引用
李晓霞,王迪,王桂臻,刘磊,李震宇,秦雪梅,李科..基于人工胃酸水解的黄芪糖指纹图谱的建立及不同种质资源黄芪鉴别[J].中国现代中药,2018,20(2):152-158,7.基金项目
国家自然科学基金资助项目(31200278) （31200278）
教育部博士点新教师类基金项目(20131401120006) （20131401120006）
