中国现代中药2018，Vol.20Issue(2)：163-168,6.DOI:10.13313/j.issn.1673-4890.20170831005
北豆根根茎中生物碱类化学成分及其生物活性研究
Alkaloids from Rhizome of Menispermum dauricum and Their Anti-inflammatory Activity
摘要
Abstract
Objective:To study the chemical structures and anti-inflammatory activity of alkaloids from Menispermum dauricum.Methods:The dried rhizome of M. dauricum were powdered and percolated with HCl(pH 3.0).The extract was concentrated under reduced pressure after adjusted to pH 7.0 and subjected to alkaline silica gel and ODS column chromatogra-phies to obtain alkaloids portion.The alkaloid-containing portion was purified by preparative HPLC,using MeOH-H2O as the mobile phase.The structures were identified by extensive spectroscopic analyses,including ESIMS and NMR.Results:Ten al-kaloids were isolated from the plant and identified as lakshminine(1),telazoline(2),daurioxoisoporphine A(3),daurioxoi-soporphine B(4),scrodentoside A(5),(-)-1,3,4-dehydrocepharanthine(6),(+)-1,3,4-dehydrocepharanthine-2′β-N-oxide(7),cissampentine A(8),cissampentine B(9),and(-)-pseudocurine(10).Compounds 6,8,9,and 10 exhib-ited anti-inflammatory activities in vitro,with IC50values of 6.0,12.0,8.5,and 9.7 μmol·L-1,respectively. Conclusion:Compounds 1-10 were obtained from the rhizomes of M.dauricum for the first time.关键词
北豆根/生物碱/抗炎活性Key words
Menispermum dauricum/alkaloids/anti-inflammatory activity引用本文复制引用
周艳丽,赵旭,李硕熙,吕红梅,王爱莉,张艳..北豆根根茎中生物碱类化学成分及其生物活性研究[J].中国现代中药,2018,20(2):163-168,6.基金项目
黑龙江中医药大学校级基金面上项目(21736) （21736）
