摘要
Abstract
Objective:To study the chemical components from the leaves of Nitraria tangutorum and its anti-inflam-mation bioactivity.Methods:The chemical components were isolated by liquid-liquid extraction,silica gel chromatography, and repeated C18semi-preparative-HPLC.Their structures were elucidated by physicochemical properties and1H-NMR and 13C-NMR data.LPS induced RAW264.7 inflammatory cell model was established to evaluate the effective inhibition of proinflamma-tory factor(TNF-α and IL-6)of compounds 5-10 from N.tangutorum.Results:Ten compounds were isolated from the ethanol extract of the leaves of N.tangutorum and their structures were identified as 3,3′,4′-tri-O-methylellagic acid(1),tricin-7-O-β-D-glucopyranoside(2),dibutyl-phthalate(3),rhamnazin(4),Isomyricadiol(5),Myricarin A(6),trihydroxy-7-megastigmen-9-one(7),1,2,3,4-Tetrahydro-1,3,4-trioxo-B-carbo1ine(8),3-hydroxy-β-carboline(9),β-carbo-line-1-carboxylic acid(10). Conclusion:All the isolates are reported from the plants of N.tangutorum.for the first time.Activity assay showed that compound 5,6,8 and 9 presented better inhibition for proinflammatory factor,the mini-mum IC50is 19.16 μmol·L-1.关键词
唐古特白刺/萜类/咔啉生物碱Key words
Nitraria tangutorum Bobr./terpenoid/carboline alkaloids
