中国现代中药2018，Vol.20Issue(2)：173-178,6.DOI:10.13313/j.issn.1673-4890.20170723002
海南裸花紫珠药材中7种成分含量分析
Analysis of Seven Components in Callicarpa nudiflora from Hainan Province
摘要
Abstract
Objective:To establish an HPLC method for the simultaneous determination of seven components and an-alyze the content variation of Callicarpa nudiflora in different origins.Methods:Analysis was performed on an Agilent TC-C18 column(250 mm×4.6 mm,5 μm)eluted with acetonitrile(A)and 0.4% phosphoric acid(B)in a gradient program.The flow rate was 1 mL·min-1,the detection wavelength was 330 nm,and the column temperature was 30 ℃,the ANOVA and PCA were used to analyze the content variance of C.nudiflora from different origins in Hainan province.Results:Under this chroma-tographic condition,the seven components showed good separation degree and regression(r >0.999 0)with linear ranges, their recoveries were in the range of 96.4%-105.5%.Obvious differences was existed in the seven components of 75 samples of C.nudiflora from Hainan province with CVs were in the range of 33.99%-83.91%,the seven component of C.nudiflora which under different climatic conditions was significantly different(P<0.05). Conclusion:The method is simple,accurate,and can be used for the quality control of C.nudiflora.The main chemical components accumulation of C.nudiflora.were greatly in-fluenced by climatic conditions in place of origin.关键词
裸花紫珠/7种成分/HPLC测定/产地/气候区Key words
Callicarpa nudiflora Hook.et Arn/seven components/HPLC-determination/origin/climatic region引用本文复制引用
谭湘杰,于福来,庞玉新,黄梅,陈振夏,杨全,李伟..海南裸花紫珠药材中7种成分含量分析[J].中国现代中药,2018,20(2):173-178,6.基金项目
海南省重大科技计划(ZDKJ2016005-03) （ZDKJ2016005-03）
