中国现代中药2018，Vol.20Issue(2)：179-183,5.DOI:10.13313/j.issn.1673-4890.20170912003
圆齿野鸦椿醇提物抗肝癌作用研究
Research on Anti-hepatoma Effect of Euscaphis konishii Hayata Ethanol Extracts
摘要
Abstract
Objective:To study the anti-hepatoma effect of the ethanol extracts of Euscaphis konishii Hayata.Methods:The E.konishii pericarps,leaves,branches and seeds were extracted by ethanol.The anti-proliferation ac-tivities in vitro were detected by MTT assay against human liver cancer cells HepG2,SMMC-7721 and BEL-7404.Flow cytome-try was used to detect the HepG2 cell apoptosis. Mice bearing H22 murine hepatoma xenograft model was established to evalu-ate the anti-tumor activity in vivo.Results:After 48 h treatment of HepG2,SMMC-7721 and BEL-7404 cells with the ethanol extracts of E.konishii pericarps,leaves,branches and seeds,MTT assay showed that the pericarps extract demonstrated the best anti-hepatoma effect in vitro,with the half inhibitory concentration(IC50)of 108.34,106.94 and 114.79 μg·mL-1.The results of flow cytometry showed that all the four extracts could obviously induce HepG2 cell apoptosis,and the pericarps ex-tract had the best effect.H22 murine hepatoma xenograft test was carried out with 200 mg·kg-1of the pericarps,leaves,bran-ches and seeds extract for 14 d by gavage administration,with the anti-tumor rate of 47.8%,44.9%,42.5% and 40.6%, respectively. Conclusion:The ethanol extract of E.konishii has obvious anti-hepatoma activity.关键词
圆齿野鸦椿/乙醇提取物/抗肝癌Key words
Euscaphis konishii Hayata/ethanol extract/anti-hepatoma引用本文复制引用
黄维,邹小兴,丁卉,梁文贤,倪林,邹双全..圆齿野鸦椿醇提物抗肝癌作用研究[J].中国现代中药,2018,20(2):179-183,5.基金项目
国家农业科技成果转化资金项目(2010GB2C00216) （2010GB2C00216）
福建省自然科学基金(13181082) （13181082）
福建农林大学科技创新专项基金项目(CXZX2016072,CXZX2016073,CXZX2016074) （CXZX2016072,CXZX2016073,CXZX2016074）
