中国现代中药2018，Vol.20Issue(2)：189-194,6.DOI:10.13313/j.issn.1673-4890.20170914002
三株甲基营养型芽孢杆菌抑菌活性物质初探
Preliminary Analysis of Active Substances from Three Bacillus methylotrophicus Strains
摘要
Abstract
Objective:Study the antagonistic active sites of Bacillus methylotrophicus QM,BM2 and HZ3 strains.Methods:Confrontation culture and oxford cup were used to verify the antifungal activity of extracts.Mycelial morphol-ogy was observed under electron microscopy.Fermentation supernatant were prepared through low temperature centrifugation and microporous filter membrane filtration.The crude extract of lipoprotein is obtained by acid precipitation method.The organic sol-vent extraction was by extracted by isometric petroleum ether,ethyl acetate and water saturated n-butanol from the supernatant, respectively.Protein crude extract was obtained by ammonium sulfate precipitation and dialysis.Results:The mycelium growth of ginseng pathogens in confrontation with three strains of B.methylotrophicus shown abnormal growth and even death.The bacte-rial fermentation broth and supernatant also have significant antagonistic activity.The extracts of lipopeptide,protein crude ex-tract and water saturated n-butanol extract in the supernatant have different inhibitory effects on ginseng pathogens.Conclusion:There are a variety of antibiotics in the fermentative of three strains of B.methylotrophicus,and it is proposed that these antibi-otics may playing antagonistic activity alone or in synergy.关键词
人参/致病菌/甲基营养型芽孢杆菌/抑菌物质Key words
Panax ginseng/phytopathogens/Bacillus methylotrophicus/antibiotics引用本文复制引用
邵天蔚,张晓云,丁万隆,王蓉,李勇..三株甲基营养型芽孢杆菌抑菌活性物质初探[J].中国现代中药,2018,20(2):189-194,6.基金项目
中医药行业科研专项(201407005) （201407005）
中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-017) （2016-I2M-3-017）
