北京中医药大学学报2018，Vol.41Issue(3)：203-209,7.DOI:10.3969/j.issn.1006-2157.2018.03.005
柴胡龙骨牡蛎汤对心肌梗死大鼠骨髓c-kit+干细胞动员的影响
Influence of Chaihu Longgu Muli Tang on mobilization of bone marrow c-kit+stem cells in rats with myocardial infarction
摘要
Abstract
Objective To investigate the influence of Chaihu Longgu Muli Tang(Chinese Thorowax Root, Bone Fossil of Big Mammals and Oyster Shell Decoction, CLMT) on the mobilization of bone marrow c-kit+stem cells in rats after myocardial infarction(MI). Methods Male SD rats were randomly divided into sham-operation group,model group and CLMT group,and each group was divided again into 3-d sub group,7-d subgroup and 14-d subgroup. The CLMT group was intragastrically given CLMT, and other groups were orally given distilled water. The heart function was reviewed by using echocardiogram, changes of myocardial pathology were reviewed after HE staining,and severity of myocardial fibrosis was observed by after Masson staining. The number of c-kit+positive cells in bone marrow and peripheral blood was detected by using flow cytometry (FCM), and number of c-kit+positive cells in MI border zone was detected by using immunohistochemistry technique. Results The heart function declined gradually along with MI time extension in model group, and difference in heart function had statistical significance compared with sham-operation group at all time points (P <0.05). The heart function trended to stable in CLMT group and was better than that in model group, and difference in LVEF and LVFS had statistical significance compared with model group at time points of 7 d and 14 d(P<0.05). Pathological staining showed that myocardial cells were in alignment without collagen fibers in sham-operation group,necrosis and fibrosis of myocardial cells in model group that aggravated gradually along with MI time extension. The severity of myocardial necrosis and fibrosis was milder in CLMT group than that in model group. The number of c-kit+positive cells in bone marrow and peripheral blood reached peak in model group and CLMT group 7 d after MI, and difference number of c-kit+positive cells had statistical significance compared with sham-operation group,and was higher in CLMT group than that in model group. The difference in number of c-kit+positive cells had statistical significance between 7-d subgroup and 14-d subgroup (P <0.05). The number of c-kit+positive cells in MI border zone was higher in model group than that in sham-operation group,and difference in number of c-kit+positive cells had statistical significance between 7-d subgroup and 14-d subgroup (P<0.05). The number of c-kit+positive cells in MI border zone was higher in CLMT group than that in model group, and difference in number of c-kit+positive cells MI border zone had statistical significance between 7-d subgroup and 14-d subgroup (P<0.05). Conclusion CLMT can improve the mobilization of bone marrow c-kit+stem cells after MI and heart function.关键词
心肌梗死/柴胡龙骨牡蛎汤/c-kit+骨髓干细胞/大鼠Key words
myocardial infarction/Chaihu Longgu Muli Tang (Chinese Thorowax Root/Bone Fossil of Big Mammals and Oyster Shell Decoction)/bone marrow c-kit+stem cells/rats分类
医药卫生引用本文复制引用
王超,杜泓森,张秀静,王昀,侯季秋,燕莎莎,杨静静,赵海滨..柴胡龙骨牡蛎汤对心肌梗死大鼠骨髓c-kit+干细胞动员的影响[J].北京中医药大学学报,2018,41(3):203-209,7.基金项目
国家自然科学基金项目(No.81373590),北京中医药大学青年教师项目(No.2017-JYB-JS-126)National Natural Science Foundation of China(No.81373590) （No.81373590）
