北京中医药大学学报2018，Vol.41Issue(3)：210-215,6.DOI:10.3969/j.issn.1006-2157.2018.03.006
调脂通脉颗粒对ApoE基因敲除小鼠血脂、RLP-C、sICAM-1、sVCAM-1的影响
Influence of Tiaozhi Tongmai Granule on blood fat, RLP-C, sICAM-1 and sVCAM-1 in ApoE-/-mice
摘要
Abstract
Objective To investigate the influence of Tiaozhi Tongmai(Fat-regulating and Vessel-freeing) Granule (TZTMG) on blood fat, triglyceride-rich remnant-like particle (RLP) and cell adhesion molecule in ApoE-/-mice. Methods After established model of atherosclerosis(AS),ApoE-/-mice(n=30) were randomly divided into model group, fenofibrate group and TZTMG group (each n =10). C57BL/6J mice (n=10) were chosen into normal group. All medicinal groups were fed with high-fat diet, meanwhile were intervened with TZTMG and fenofibrate. The indexes of triglyceride-rich RLP (RLP-C) and 4 items of blood fat were detected detector after 12 weeks. The levels of serum intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) were detected by using ELISA. The aortic root was collected to prepare paraffin sections for observing morphological changes af-ter HE staining. Results Since the 4thweek, mouse weight decreased in TZTMG group and fenofibrate group compared with model group. The levels of serum RLP-C, TC, TG, HDL-C and LDL-C increased in model group,fenofibrate group and TZTMG group compared with normal group(P<0.05). The lev-els of serum RLP-C and TG decreased in fenofibrate group and TZTMG group compared with model group (P<0.05). The levels of ICAM-1 and VCAM-1 increased significantly in model group, fenofibrate group and TZTMG group compared with normal group (P <0.05). The levels of serum ICAM-1 and VCAM-1 decreased in fenofibrate group and TZTMG group compared with model group (P <0.05). Comparison in VCAM-1 among normal group,fenofibrate group and TZTMC group had no statistical sig-nificance. HE staining showed there were thickened aortic intima and vulnerable plaques in model group, and thickened aortic intima and stable plaques in TZTMG group. Conclusion TZTMG can decrease the expression of adhesion molecule and delay AS development through reducing RLP-C level.关键词
血脂/富含甘油三酯脂蛋白残粒/动脉粥样硬化/ApoE基因敲除小鼠Key words
blood fat/triglyceride-rich remnant-like particle/atherosclerosis/ApoE-/-mice分类
医药卫生引用本文复制引用
李璐,郭杨志,杨惠民,钱丽超,史成,王淑艳,廖磊..调脂通脉颗粒对ApoE基因敲除小鼠血脂、RLP-C、sICAM-1、sVCAM-1的影响[J].北京中医药大学学报,2018,41(3):210-215,6.基金项目
国家自然科学基金项目(No.81503503)National Natural Science Foundation of China(No.81503503) （No.81503503）
