北京中医药大学学报2018，Vol.41Issue(3)：227-234,8.DOI:10.3969/j.issn.1006-2157.2018.03.007
电针对高血脂合并脑缺血大鼠血脂和脑梗死体积的影响及其机制探讨
Influence of electroacupuncture on blood fat and volume of cerebral infarction and mechanism exploration in rats with hyperlipidemia complicated by cerebral ischemia
摘要
Abstract
Objective To discuss the influence of electroacupuncture (EA) on blood fat and volume of cerebral infarction in rats with hyperlipidemia complicated by cerebral ischemia,and explore the mecha-nism. Methods SD rats (n=78) were randomly divided into normal group (n=6), and model group, sham-operation group,EA group 1 and EA group 2(each n=18). Except of normal group,other groups were given high fat diet for 6 weeks for establishing hyperlipidemia model. After 6 weeks, EA group 1 was given EA in bilateral Fenglong(ST40) once a day for 7 d. On the 50thd,the model of middle cere-bral artery occlusion (MCAO) was established in alll groups except of rormal group,and then EA group 1 and EA group 2 were given EA in Baihui (GV20) and bilateral Fenglong (ST40) once a day. The neurological function and volume of cerebral infarction were detected 1 d after treatment. The levels of to-tal cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL) were detected 1 d,7 d and 14 d after treatment. The protein expressions of β-NGF and TrkA were detected by using Western blotting assay in diseased brain. Results The levels of TC, G and LDL increased and level of HDL decreased after treatment (P <0.01, P <0.05). Compared with model group,level of TG decreased in EA groups after treatment for 7 d (P<0.01), and level of LDL de-creased in EA group 1 after treatment for 14 d (P <0.05), which had no significant in EA group 2. Compared with model group,neurological deficit score (NDS) decreased in EA group 1 after treatment for 1 d (P<0.05),and left volume of cerebral infarction decreased in EA groups (P<0.01), which was smaller in EA group 1 than that in EA group 2(P<0.05). Compared with normal group, expres-sions of β-NGF and TrkA had an ascending trend in model group after treatment for 1 d,and recover to normal after treatment for 7 d. Compared with model group,expressions of β-NGF and TrkA had an as-cending trend in EA group 1 and continuously increased after treatment for 14 d,which was more signifi-cantly in EA group 1. Conclusion EA can improve neurological function,reduce volume of cerebral in-farction and promote expressions of β-NGF and TrkA through regulating blood fat level in hyperlipidemia stage,and its regulation effect is better than that of acupuncture after cerebral ischemia. The possible mechanism is related to that GV20 and ST40 can cmprove expressions of β-NGF and TrkA.关键词
电针/高脂血症/脑缺血/β-神经生长因子/络氨酸蛋白激酶/大鼠Key words
electroacupuncture/hyperlipidemia/cerebral ischemia/β-nerve growth factor/tyrosine ki-nase A/rats分类
医药卫生引用本文复制引用
于若凡,于雪,任秀君..电针对高血脂合并脑缺血大鼠血脂和脑梗死体积的影响及其机制探讨[J].北京中医药大学学报,2018,41(3):227-234,8.基金项目
国家自然科学基金项目(No.81470200)National Natural Science Foundation of China(No.81470200) （No.81470200）
