重庆医学2017，Vol.46Issue(35)：4901-4903,4907,4.DOI:10.3969/j.issn.1671-8348.2017.35.002
聚乙烯醇-卟啉新型纳米颗粒的制备及其光热特性
Preparation of new type polyvinyl alcohol-porphyrin nanoparticles and its photothermal properties
摘要
Abstract
Objective To analyze the physicochemical properties and photothermal properties after introducing a certain proportion of phaeophorbide into polyvinyl alcohol-lipoic acid polymer.Methods The "one-step" method was adopted to synthesize the PVA27K-4 ％LA-10 ％ Pheide (PLP) polymer.The laser particle diameter tester was used to detect the particle size,polydispersity index and ζ potential of PLP nanoparticle (PLPN);the morphology was observed under transmission electron microscope (TEM);the in vitro fluorescent and photothermal properties were investigated by using spectrophotometric method and infrared thermal imager;the ovarian-cancer nude mice models were established and the preliminary in vivo experiment evaluation was performed.Results The synthetic PLP polymer could self-assembly form spherical PLPN in aqueous environment,which showed the tightly spherical structure under TEM.The average particle diameter,polydispersity index and ζ potential were (22.92 ± 9.00)nm,(0.117±0.056) and (-25.43±0.77)mV respectively.The fluorescence intensity of PLPN in PBS solution was significantly lower than that in SDS solution (P＜0.05).However,the temperature of the PBS solution of PLPN was significantly higher than that of the SDS solution after exposure to the near infrared light (P＜0.05).In the model of tumor-bearing nude mice,PLPN could targetaccumulated in the tumor site.The temperature in the tumor site was significantly increased after exposure to the near infrared light,which could kill tumor cells.Conclusion PLPN could emit red fluorescence,and could target-accumulated in tumor site and conduct the in vivo image,which has significant photothermal conversion properties in vivo and in vitro,and can effectively inhibit the tumor growth in vivo.关键词
聚乙烯醇/脱镁叶绿酸/纳米粒子/光热转换/卵巢肿瘤Key words
polyvinyl alcohol/pheophorbide/nanoparticles/photothermal conversion/ovarian neoplasms分类
医药卫生引用本文复制引用
李军果,王全晖,史海江,宋宗昌,罗焱..聚乙烯醇-卟啉新型纳米颗粒的制备及其光热特性[J].重庆医学,2017,46(35):4901-4903,4907,4.基金项目
总后卫生部青年培育项目(14QNP030). （14QNP030）
