重庆医学2018，Vol.47Issue(9)：1158-1160,1164,4.DOI:10.3969/j.issn.1671-8348.2018.09.003
抑制DNA-PKcs对放射治疗后食管鳞癌细胞系EC109自噬蛋白表达和增殖的影响
Effects of blocking DNA-PKcs on autophagy protein expression and proliferation in esophageal squamous cell carcinoma cell line EC109 after irradiation
摘要
Abstract
Objective To study the effect of DNA-PKcs blocking on the expression of autophagic proteins,and proliferation of esophageal squamous cell carcinoma cells(EC109)after irradiation(X-Ray).Methods NU7441 was used to inhibit DNA-PKcs and X-Ray radiation treatment were used to treat EC109 cells.Th experiment was divided into 4 groups,including control group, NU7441 group,X-Ray group,X-Ray+ NU7441 group.The expressions of autophagy protein Beclin-1 and LC3B were detected by Western blot.Apoptosis was detected by flow cytometry.MTT assay was used to detect cell proliferation.Results The expression of p-DNA-PKcs in EC109 cells was decreased after NU7441 treatment and increased after X-ray irradiation.Compared with untreat-ed cells(control group),the expressions of both Beclin-1 and LC3B in X-Ray+NU7441 group were increased.Compared with the X-Ray group,the expression of Beclin-1 in the X-Ray+NU7441 group was increased.Compared with the control group,the apoptotic rate of EC109 cells in the X-Ray group and X-Ray+NU7441 group was significantly increased,the difference was statistically significant(P<0.05).Compared with the X-Ray group the apoptosis rate in the X-Ray-NU7441 group was significantly increased.The MTT results showed that compared with the control group,the proliferation in the X-Ray group and X-Ray+NU7441 group was significantly inhibited, the difference was statistically significant(P<0.05).Conclusion NU7441 inhibits the expression of DNA-PKcs protein in EC109 cells, which could promote the expressions of autophagy protein Beclin-1 and LC3B,promotes apoptosis and inhibits cell proliferation.关键词
DNA-PKcs/自噬蛋白/细胞增殖/食管肿瘤/癌,鳞状细胞Key words
DNA-dependent protein kinase catalytic/autophagy protein/cell proliferation/esophageal neoplasms/carcinoma/squamous cell分类
医药卫生引用本文复制引用
孙佳,王健,赵晓静,袁翎,李雨濛,李南,张中冕,王豪勋,韩娜,马军..抑制DNA-PKcs对放射治疗后食管鳞癌细胞系EC109自噬蛋白表达和增殖的影响[J].重庆医学,2018,47(9):1158-1160,1164,4.基金项目
河南省科技厅科技攻关项目(152300410164). （152300410164）
