重庆医学2018，Vol.47Issue(9)：1161-1164,4.DOI:10.3969/j.issn.1671-8348.2018.09.004
IgE高亲和力受体蛋白的制备及其生物功能的鉴定
Preparation of IgE high affinity receptor protein and its biological function identification
徐婷 1张强 1喻荷莲 1王世春 1徐小敏 1易中梅 1蒋天伦1
作者信息
- 1. 陆军军医大学西南医院输血科,重庆400038
- 折叠
摘要
Abstract
Objective To prepare human alpha segment of high affinity IgE receptor(FcεRIα)protein by genetic engineering technology and to identify its biological function for laying the foundation for further researching the role of FcεRIα in allergic disea-ses.Methods The human FcεRIα gene was obtained by the PCR based accurate synthesis(PAS)method and the prokaryotic ex-pression vector pET-28a(+)was constructed.The FcεRIα was expressed at low temperature induction and the recombinant protein was purified by His tag.The biological function of recombinant human FcεRIα protein was identified by ELISA.Results The hu-man FcεRIα gene was amplified by PAS with a size of approximately 560 bp.The pET-FcεRIα plasmid was correct through the double enzyme digestion and sequencing identification.The human FcεRIα with a molecular weight of approximately 22 000 was in-duced and purified.The recombinant human FcεRIα could effectively detect human serum anti-FcεRIα autoantibody and could com-bined with serum IgE antibodies with high efficiency.Conclusion Human FcεRIα protein is successfully prepared,which prelimina-rily has the ability for detecting the human serum anti-FcεRIα autoantibodies and IgE antibodies,and provides a favorable practical base for further study.关键词
IgE高亲和力受体/IgE抗体/抗FcεRIα自身抗体/酶联免疫吸附测定Key words
IgE high affinity receptor/IgE antibody/anti-FcεRIα autoantibody/enzyme-linked immunosorbent assay分类
医药卫生引用本文复制引用
徐婷,张强,喻荷莲,王世春,徐小敏,易中梅,蒋天伦..IgE高亲和力受体蛋白的制备及其生物功能的鉴定[J].重庆医学,2018,47(9):1161-1164,4.
