重庆医学2018，Vol.47Issue(9)：1165-1168,4.DOI:10.3969/j.issn.1671-8348.2018.09.005
Map3k1基因下调对B6小鼠眼睑角质形成细胞增殖和迁移能力的影响
Effect of Map3k1 gene down regulation on proliferation and migration abilities of B 6 mouse eyelid keratinocytes
摘要
Abstract
Objective To study the effect of targeted inhibition of mitogen-activated protein kinase kinase kinase 1(Map3k1) gene on the cell proliferation and migration abilities of B6 mouse eyelid keratinocytes.Methods An artificial microRNA(amiRNA) interference vector targeting silent Map3k1 gene was constructed in vitro in the test.Lipofectamin 2000 was used to transfect the B6 mouse eyelid keratinocytes(the Ctrl Map3k1 group was transfected with empty vector,while the Map3k1 amiRNA-3 group was transfected with Map3k1 amiRNA-3 interference vector).The Map3k1 mRNA and protein expression levels were respectively de-tected by real-time PCR and Western-blot for determining the interference efficiency.The B6 mouse eyelid keratinocytes prolifera-tion level was detected by MTT.The migration ability of keratinocytes was detected by the scratch experiment.Results After the keratinocytes were transfected with Map3k1 amiRNA interference vector,the levels of Map3k1 mRNA and protein were effectively inhibited,and the interference efficiency was up to 70%(P<0.05).The proliferation level of keratinocytes in the Map3k1 amiRNA-3 group was lower than that in the Ctrl Map3k1 group(P<0.05).The migratory ability of keratinocytes in the Map3k1 amiRNA-3 group was also significantly lower than that in the Ctrl Map3k1 group(P<0.05).Conclusion Targeted inhibition of Map3k1 gene expression in B6 mouse eyelid keratinocytes significantly inhibits cell proliferation and migration,thus influence the cellular bi-ological behaviors.关键词
Map3k1/角质形成细胞/RNA干扰/细胞增殖/迁移能力Key words
Map3k1/keratinocyte/RNA interference/cell proliferation/migretion ability分类
医药卫生引用本文复制引用
景瑾,刘莉莉,尤杰,夏婷婷,张进,刘春,朱顺星..Map3k1基因下调对B6小鼠眼睑角质形成细胞增殖和迁移能力的影响[J].重庆医学,2018,47(9):1165-1168,4.基金项目
江苏省高等学校大学生创新创业训练计划项目(201513993008Y) （201513993008Y）
南通市市级科技计划项目(YYZ16044). （YYZ16044）
