重庆医学2018，Vol.47Issue(9)：1173-1175,3.DOI:10.3969/j.issn.1671-8348.2018.09.007
MiR-650通过靶向ING4促进骨肉瘤细胞增殖的研究
Study on miR-650 for promoting proliferation of osteosarcoma cells via targeting ING4
摘要
Abstract
Objective To investigate the expression of miR-650 in osteosarcoma tissue and cell lines and its action mechanism in the osteosarcoma formation.Methods The realtime fluorescence quantitative PCR was used to detect and compare the expres-sions of miR-650 between osteosarcoma tissue and normal tissue or between osteosarcoma cell lines(MG63)and normal human os-teoblast cell lines(hFOB1.19);the MTT experiment was used to investigate the proliferation situation in different groups;Western blot was used to detect the ING4 protein expression.Results The expression of miR-650 in osteosarcoma tissue and MG63 cells was higher than that in normal tissue and human osteoblast cell;after inhibiting miR-650 expression,the proliferation ability of MG63 was significantly decreased.Moreover the expression of inhibitor of growth 4(ING4)was significantly increased when miR-650 was reduced,ING4 mRNA of negative control group,scramble group and miRNA group were 1.00 ± 0.16,1.08 ± 0.14 and 5.35 ± 0.32 respectively;the ING4 protein expressions were 0.62 ± 0.06,0.59 ± 0.12 and 2.45 ± 0.20 respectively;compared with negative control group,the difference was statistically significant(P<0.05);but after suppressing this elevation,the proliferation a-bility of MG63 cells had a certain recovery.Conclusion MiR-650 promotes MG63 proliferation via reducing ING4 expression,which suggests that miR-650 could be a new target of treating osteosarcoma.关键词
骨肉瘤/MG63/miR-650/调节生长抑制因子4Key words
osteosarcoma/MG63/miR-650/inhibitor of growth 4分类
医药卫生引用本文复制引用
陈贤明,张森,王爱民,王子明..MiR-650通过靶向ING4促进骨肉瘤细胞增殖的研究[J].重庆医学,2018,47(9):1173-1175,3.基金项目
陆军军医大学大坪医院野战外科研究所1135人才基金. （）
