重庆医学2018，Vol.47Issue(9)：1176-1178,1182,4.DOI:10.3969/j.issn.1671-8348.2018.09.008
白藜芦醇后处理通过PI3K/Akt/Nfr2上调HO-1对大鼠心肌I/R的保护研究
Study on protective effect of resveratrol post-treament on rat myocardial I/R injury through up-regulating HO-1 via PI3K/Akt/Nfr2
邓颖 1张雄 2胡红3
作者信息
- 1. 重庆医科大学附属第一医院第一分院心内科 400011
- 2. 重庆医科大学神经科学研究中心 400016
- 3. 重庆医科大学附属第一医院第一分院肾内科 400011
- 折叠
摘要
Abstract
Objective To observe the effect of resveratrol(Res)on myocardial ischemia/reperfusion(I/R)injury in rats and to explore its potential mechanisms.Methods Sixty male SD rats were selected and divided into the sham operation group,I/R group,I/R+ Res group and I/R+ Res +LY294002(I/R+Res+LY)group.The rat myocardial I/R injury model was established by the ligation of left anterior descending coronary artery for 30 min and then by reperfusion for 2 h,and the injection of Res(20 mg/kg)or LY294002(5 mg/kg)was given via sublingual vein at 1 min before reperfusion.The levels of malondialdehyde(MDA) and superoxide dismutase(SOD)in plasma and myocardial tissue,and level of heme oxygenase 1(HO-1)in myocardial tissues were determined in each group;Western blot was used to detect the expression level change of PI3K,Akt(p-Akt),Nrf2 and HO-1 protein in myocardial tissue.Results Compared with the I/R group,the plasma and myocardial MDA levels in I/R+ Res group were re-duced,the SOD activity level was elevated,and the activity and expression of HO-1 was enhanced(P<0.05);compared with the I/R gourp,the expression levels of PI3K,Akt(p-Akt),nrf2 and HO -1 protein levels in myocardial tissue of I/R+ Res group were increased(P<0.05).But these effects in the I/R+Res+LY group could be partially eliminated by PI3K/Akt inhibitor LY294002. Conclusion Resveratrol post-treatment can enhance the activity and expression of HO-1 protein through up-regulating the activity of PI3K/Akt/Nfr2,and then promotes the activity and expression of HO-1,thus plays the HO-1 anti-oxidative role,and alleviates myocardial I/R injury.关键词
白藜芦醇/心肌缺血/再灌注损伤/血红素加氧酶-1/PI3K/Akt/Nfr2/大鼠,Sprague-DawleyKey words
resveratrol/myocardial ischemia/ischemia-reperfusion injury/heme oxygenase-1/PI3K/Akt/Nfr2/rats/Sprague-Dawley分类
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邓颖,张雄,胡红..白藜芦醇后处理通过PI3K/Akt/Nfr2上调HO-1对大鼠心肌I/R的保护研究[J].重庆医学,2018,47(9):1176-1178,1182,4.
