南方医科大学学报2018，Vol.38Issue(3)：243-250,8.DOI:10.3969/j.issn.1673-4254.2018.03.02
白杨素通过JAK-STATs信号通路抑制内毒素诱导的巨噬细胞炎症反应
Chrysin inhibits lipopolysaccharide-induced inflammatory responses of macrophages via JAK-STATs signaling pathway
摘要
Abstract
Objective To investigate the mechanism of chrysin in regulating lipopolysaccharide(LPS)-induced inflammation in RAW264.7 cells.Methods RAW264.7 cells were treated with different concentrations(0,5,10,20,40,60,80,100,150,and 200 μg/mL)of chrysin for 24 h,and the cell viability was measured using CCK-8.RAW264.7 cells were pre-treated with 10,30,or 60 μg/mL chrysin for 2 h before stimulation with LPS for different times.The levels of TNF-α,IL-6 and MCP-1 were detected by ELISA, and Western blotting was used to detect the phosphorylation of JAK-1, JAK-2, STAT-1 and STAT-3. The level of reactive oxygen species in RAW264.7 cells was detected by CM-H2DCFDA fluorescence probe.The effect of ROS on LPS-induced JAK-STATs signal and the inflammatory response of RAW264.7 cells was detected by ROS scavenger NAC. The transcription factors STAT-1 and STAT-3 nuclear translocation were observed by laser confocal microscopy. Results Chrysin below 60 μg/mL did not significantly affect the viability of RAW264.7 cells.At 10,30,and 60 μg/mL,chrysin dose-dependently inhibited the expression of iNOS induced by LPS. Chrysin treatment also inhibited LPS-induced phosphorylation of JAK-STATs, nuclear translocation of STAT1 and STAT3, release of TNF-α, IL-6 and MCP-1, and the production of ROS in RAW264.7 cells;ROS acted as an upstream signal to mediate the activation of JAK-STATs signaling pathway.Conclusion Chrysin blocks the activity of JAK-STATs mediated by ROS to inhibit LPS-induced inflammatory response in RAW264.7 cells.关键词
白杨素/活性氧簇/RAW264.7细胞/JAK-STATs信号通路Key words
chrysin/reactive oxygen species/RAW264.7 cells/JAK-STATs引用本文复制引用
齐世美,李强,姜琦,戚之琳,章尧..白杨素通过JAK-STATs信号通路抑制内毒素诱导的巨噬细胞炎症反应[J].南方医科大学学报,2018,38(3):243-250,8.基金项目
国家自然科学基金(31301171,81601380) （31301171,81601380）
安徽省自然科学基金面上项目(1508085MH149) （1508085MH149）
芜湖市科技局产学研合作专项基金(2013cxy04) （2013cxy04）
高等学校省级优秀青年人才基金重点项目(2013SQRL055ZD) （2013SQRL055ZD）
活性生物大分子研究安徽省重点实验室项目(1306C083008) （1306C083008）
安徽高校自然科学研究项目重大项目(KJ2016SD59) （KJ2016SD59）
安徽省优秀青年人才支持计划重点项目(gxyqZD2016173) （gxyqZD2016173）
皖南医学院博士科研启动基金项目(201223) （201223）
2017年度皖南医学院重点科研项目(WK2017Z07) Supported by National Natural Science Foundation of China(31301171, 81601380). （WK2017Z07）
