南方医科大学学报2018，Vol.38Issue(3)：268-273,6.DOI:10.3969/j.issn.1673-4254.2018.03.05
p38MAPK信号通路在鼠伤寒沙门菌spvB基因影响Henle-407细胞自噬中的作用
Role of p38MAPK signaling pathway in autophagy of Henle-407 cells induced by spvB of Salmonella typhimurium
摘要
Abstract
Objective To investigate the role of p38MAPK signaling pathway in autophagy of intestinal epithelial cells induced by spvB of S.typhimurium.Methods Henle-407 cells in exponential growth were infected with wild-type S.typhimurium strain STM-211(with spvB gene),spvB mutated strain STM-ΔspvB,or with Δ spvB-complemented strain STM-c-spvB after treatment of the cells with the p38MAPK inhibitor SB203580. At different time points of co-culture, the cells were collected and the intracellular bacteria were counted. Western blotting was performed to detect the expressions of phosphorylated p38 and autophagy-related proteins LC3 and p62;immunofluorescence staining was used to observe the expression and distribution of LC3.Results At 1,2 and 4 h after the infection,the phosphorylation levels of p38 in STM-211 group and STM-c-spvB group were significantly lower than that in STM-ΔspvB group(P<0.05).At 2 and 4 h of co-culture,the intracellular bacterial counts were significantly greater in STM-211 and STM-c-spvB infection groups than in STM-ΔspvB group(P<0.05).Pretreatment with p38 inhibitor SB203580 did no significantly affect the expression levels of LC3 II or P62 in STM-211 and STM-c-spvB groups, but caused significant reduction in their expressions in STM-ΔspvB group at 1 h (P<0.05), and such changes were more obvious at 3 h(P<0.05).Conclusion The inhibitory effect of spvB gene on autophagy in intestinal epithelial cells is related with the negative regulation of p38MAPK signaling pathway.关键词
鼠伤寒沙门菌/spvB基因/p38MAPK信号通路/自噬Key words
Salmonella typhimurium/spvB gene/p38MAPK signaling pathway/autophagy引用本文复制引用
赖华毅,陈强,李红,朱春晖,易丽君,周菁,胡清华,余晓君..p38MAPK信号通路在鼠伤寒沙门菌spvB基因影响Henle-407细胞自噬中的作用[J].南方医科大学学报,2018,38(3):268-273,6.基金项目
国家自然科学基金(81460117) Supported by National Natural Science Foundation of China(81460117). （81460117）
