南方医科大学学报2018，Vol.38Issue(3)：324-328,5.DOI:10.3969/j.issn.1673-4254.2018.03.13
弱精子症患者精子中DKKL1的表达
Expression of DKKL1 in spermatozoa of men with asthenospermia
摘要
Abstract
Objective To compare the expression of DKKL1 in ejaculated spermatozoa of normal fertile men and men with asthenospermia and investigate the role of DKKL1 in the pathogenesis of asthenospermia. Methods The characteristics of semen samples collected from normal fertile men and men with asthenospermia were analyzed using computer-assisted sperm analysis according to WHO criteria.The ejaculated sperms were isolated by Percoll discontinuous density gradients to detect the expression of DKKL1 mRNA and protein using real-time PCR and Western blotting. Results The expression of DKKL1 mRNA was significantly down-regulated by 11.1 times in asthenospermic men as compared with that in normal fertile men (P<0.01). Western blotting showed that the expression of DKKL1 protein was down-regulated by 2.4 times in asthenospermic men compared to normal fertile men. Conclusion The expression of DKKL1, which may play an important role in sperm motility,is significantly decreased in ejaculated spermatozoa of men with asthenospermia.关键词
DKKL1/男性不育/弱子精症Key words
DKKL1/male infertility/asthenospermia引用本文复制引用
颜秋霞,马义,陈润强,周秀琴,乔静,冼英杰,冯玲,陈彩蓉..弱精子症患者精子中DKKL1的表达[J].南方医科大学学报,2018,38(3):324-328,5.基金项目
国家自然科学基金(81373314) （81373314）
广东省医学科研基金(B2014426) （B2014426）
清远市科技计划项目(2014A002,2014B008) （2014A002,2014B008）
广州市科技计划项目(201707010245,201704020117) （201707010245,201704020117）
广东省自然科学基金(2015A030313333) Supported by National Natural Science Foundation of China(81373314). （2015A030313333）
