南方医科大学学报2018，Vol.38Issue(3)：334-339,6.DOI:10.3969/j.issn.1673-4254.2018.03.15
大肠杆菌胞外囊泡对人神经母细胞瘤SK-N-SH细胞增殖、凋亡和迁移的影响
Effect of outer membrane vesicles derived from Escherichia coli on proliferation, apoptosis and migration of human neuroblastoma SK-N-SH cells in vitro
摘要
Abstract
Objective To investigate the effect of outer membrane vesicles derived from Escherichia coli on proliferation, apoptosis and migration of human neuroblastoma SK-N-SH cells in vitro.Methods The outer membrane vesicles(OMVs)were obtained from wild-type Escherichia coli with ultracentrifugation method,and the morphology of the OMVs was observed by transmission electron microscopy and the vesicle diameter was determined using MALVERN ZEN3690. Human neuroblastoma SK-N-SH cells were treated with the OMVs at low(100 μg/mL),moderate(500 μg/mL)and high(1000 μg/mL) doses, and 24, 48 and 72 h later, the cell proliferation activity was detected with MTT assay. The expressions of apoptosis-related marker caspase-3 was detected using Western blotting,and TUNEL assay was performed to detect the cell apoptosis. The migration capacity of SK-N-SH cells was evaluated using Transwell migration assay.Results The isolated OMVs showed a circular or elliptical hollow structure with double-layer membrane and a diameter range of 30-450 nm. Compared with the control cells, SK-N-SH cells treated with the OMVs showed significantly lowered cell proliferation capacity with enhanced expression of caspase-3. Treatment of the cells with the OMVs resulted in increased cell apoptosis and significantly lowered migration capacity (P<0.05). Conclusion The OMVs derived from Escherichia coli can produce cytotoxicity against SK-N-SH cells and might serve as a therapeutic agent for refractory neuroblastoma.关键词
SK-N-SH细胞/大肠杆菌胞外囊泡/增殖/凋亡/迁移Key words
neuroblastoma/SK-N-SH cells/outer membrane vesicles/Escherichia coli/cell proliferation/apoptosis/migration引用本文复制引用
唐波,何大维,李典,郭文浩,张丹,魏光辉..大肠杆菌胞外囊泡对人神经母细胞瘤SK-N-SH细胞增殖、凋亡和迁移的影响[J].南方医科大学学报,2018,38(3):334-339,6.基金项目
国家自然科学基金(81370701) （81370701）
重庆市社会事业与民生保障科技创新专项(cstc2017shmsA130103) Supported by National Natural Science Foundation of China(81370701). （cstc2017shmsA130103）
