南方医科大学学报2018，Vol.38Issue(3)：340-345,6.DOI:10.3969/j.issn.1673-4254.2018.03.16
人恶性脑膜瘤原代细胞的培养及小鼠颅内原位成瘤模型的建立
Primary culture of human malignant meningioma cells and its intracranial orthotopic transplantation in nude mice
摘要
Abstract
Objective To obtain stable primary cultures of human malignant meningioma cells and establish an intracranial in-situ tumor model in nude mice. Methods Ten surgical specimens of highly suspected malignant meningioma were obtained with postoperative pathological confirmation. Primary malignant meningioma cells were cultured from the tissues using a modified method and passaged.After identification with cell immunofluorescence,the cultured cells were inoculated into the right parietal lobe of 6 nude mice using stereotaxic apparatus and also transplanted subcutaneously in another 6 nude mice. The nude mice were executed after 6 weeks, and HE staining and immunohistochmistry were used to detect tumor growth and the invasion of the adjacent brain tissues. Results The primary malignant meningioma cells were cultured successfully, and postoperative pathology reported anaplastic malignant meningioma. Cell immunofluorescence revealed positivity for vimentin and EMA in the cells,which showed a S-shaped growth curve in culture.Flow cytometry revealed a cell percentage in the Q3 area of(95.99±2.58)%.Six weeks after transplantation,tumor nodules occurred in the subcutaneous tumor group,and the nude mice bearing the in situ tumor showed obvious body weight loss.The xenografts in both groups contained a mean of (36±5.35)% cells expressing Ki-67,and the intracranial in situ tumor showed obvious invasion of the adjacent peripheral brain tissues.Conclusion We obtained stable primary cultures of malignant meningioma cells and successfully established a nude mouse model bearing in situ human malignant meningioma.关键词
恶性脑膜瘤/原代细胞培养/细胞鉴定/颅内成瘤模型/Ki-67Key words
malignant meningiomas/primary cells culture/cell identification/intracranial tumor model/Ki-67引用本文复制引用
胡梅新,刘家乐,陈宣伯,徐安琪,舒松仁,汪潮湖,刘忆..人恶性脑膜瘤原代细胞的培养及小鼠颅内原位成瘤模型的建立[J].南方医科大学学报,2018,38(3):340-345,6.基金项目
广州市科技计划项目(201707010149) （201707010149）
广东省自然科学基金博士启动项目(2016A030310377) （2016A030310377）
南方医科大学南方医院院长基金青年项目(2015C018) （2015C018）
南方医科大学南方医院院长基金专项项目(2017Z009) （2017Z009）
