南方医科大学学报2018，Vol.38Issue(3)：346-352,7.DOI:10.3969/j.issn.1673-4254.2018.03.17
少阳主骨方介导p19Arf-p53-p21Cip1信号通路调控食蟹猴关节软骨退变的机制
Shaoyangzhugu Formula regulates p19Arf-p53-p21Cip1signaling pathway to ameliorate cartilage degeneration in aged cynomolgus monkeys with knee osteoarthritis
摘要
Abstract
Objective To study the effect of Shaoyangzhugu(SYZG)Formula(a formula consisting of 9 traditional Chinese drugs) in delaying the degeneration of articular cartilage and the role p19Arf-p53-p21Cip1signaling pathway in mediating this effect. Method Thirteen aged cynomolgus monkeys with degenerative knee joints were selected based on X-ray findings,and one of them was randomly selected for pathological observation. The other monkeys were randomized equally into SYZG Formula group (treated with SYZG decoction), ammonia moxime group and saline group. All the monkeys were sacrificed after 8 weeks of treatment with intragastric administration of the drugs or saline.The pathology in the knee joint articular cartilage was observed and the mRNA and protein expressions of p19Arf,p53,and p21Cip1in the articular cartilage were detected using RT-qPCR and Western blotting.Results The pathological findings of the articular cartilage in old cynomolgus monkeys were consistent with the characteristics of knee osteoarthritis(KOA).Mankin scores of the cynomolgus monkeys were 7.38±0.52 in SYZG Formula group, 7.88±0.83 in ammonia moxime group, and 8.38±0.74 in saline group, showing a significant difference between SYZG Formula group and saline group(P<0.05).The expressions of p19Arf,p53,and p21Cip1were the lowest in SYZG Formula group and the highest in saline group with significant differences among the 3 groups (P<0.05). Conclusion SYZG Formula can delay chondrocyte senescence by regulating p19Arf-p53-p21Cip1signaling pathway to delay articular cartilage degeneration in aged cynomolgus monkeys.关键词
少阳主骨/食蟹猴KOA模型/细胞衰老/p19Arf-p53-p21Cip1信号通路Key words
Shaoyangzhugu Formula/cynomolgus monkeys/knee osteoarthritis/cell senescence/p19Arf-p53-p21Cip1signaling pathway引用本文复制引用
周鑫,张磊,扶世杰,刘刚,郭晓光,易刚,刘洋,汪国友..少阳主骨方介导p19Arf-p53-p21Cip1信号通路调控食蟹猴关节软骨退变的机制[J].南方医科大学学报,2018,38(3):346-352,7.基金项目
四川省科技支撑计划项目课题项目(2014SZ0185) （2014SZ0185）
泸州市科技计划项目课题项目(2013LZLY-K59|) （2013LZLY-K59|）
西南医科大学-西南医科大学附属中医医院联合专项项目(2016-4-4) （2016-4-4）
2016年泸州市-西南医科大学科技战略合作项目(2016LZXNYD-J08) （2016LZXNYD-J08）
泸州市院士工作站在建项目(20180101) （20180101）
