南方医科大学学报2018，Vol.38Issue(3)：363-368,6.DOI:10.3969/j.issn.1673-4254.2018.03.20
双层人工皮对猪急性皮肤缺损创面的疗效评价及作用机制
Efficacy of dermal scaffold for promoting repair of acute full-thickness skin defects in pigs
摘要
Abstract
Objective To investigate the efficacy of Lando?dermal scaffold for promoting repair of acute full-thickness skin defects in pigs and explore the possible mechanism. Methods Three 5 cm×5 cm full-thickness skin defects were created on the left dorsal skin(control group)and another 3 on the right dorsal skin(treatment group)of each of 6 Tibetan pigs.The wounds in the treatment group were covered with a bilayer artificial skin(Lando)and the control wounds with vaseline gauze.In both groups, autogenous split-thickness skin were grafted to the wounds 2 weeks later (with the silicone rubber membrane removed before grafting in the treatment group).At 3 days and 2 and 10 weeks after the injury,the wounds were assessed for general condition and contraction,and tissue samples were collected from the wounds to examine the expressions of α-smooth muscle actin(α-SMA)and transforming growth factor-β1(TGF-β1)using immunohistochemistry and the expressions of MMP-1 and TIMP-1 mRNA using RT-PCR. Results At 3 days after the injury, the wounds in the 2 groups showed no significant differences in the results of any examinations.At 2 weeks after the injury,the wounds in the treatment group showed rich and more smooth granulation tissues with more regular wound edges compared with the control wounds.At 2 and 10 weeks after the injury, the wound contraction rates in the treatment group were (30.5 ± 3.4)% and (39.2 ± 2.8)%, respectively, significantly lower than the rates of(51.8±2.6)%(t=-29.840,P=0.000)and(60.7±2.2)%(t=-50.213,P=0.000)in the control group.At 2 weeks,the wound tissues in the treatment group expressed significantly higher levels of α-SMA(t=15.921,P=0.000)and TGF-β1(t=29.995, P=0.000) than the control wounds, but at 10 weeks, the expressions of α-SMA(t=-41.823, P=0.000) and TGF-β1 (t=-99.777, P=0.000) in the treatment group were significantly lower than those in the control group. Compared with those in the control group, the expression of MMP-1 mRNA in the treatment group was significantly lower at 2 weeks (t=-45.412, P=0.000) but significantly higher at 10 weeks (t=78.769, P=0.000), and the expression of TIMP-1 mRNA in the treatment group was significantly lower both at 2 weeks (t=-27.064, P=0.000) and at 10 weeks (t=-40.535, P=0.000). Conclusions Lando?dermal scaffold can promote granulation tissue growth possibly in relation with increased TGF-β1 and decreased MMP-1 expression in the wounds.This scaffold material also reduces wound contraction and lessens scar hyperplasia and contracture after wound healing,probably as a result of decreased α-SMA, TGF-β1, and TIMP-1 and increased MMP-1 expressions.关键词
人工皮肤/皮肤移植/创面愈合Key words
artificial skin/skin graft/wound healing引用本文复制引用
邱学文,王甲汉..双层人工皮对猪急性皮肤缺损创面的疗效评价及作用机制[J].南方医科大学学报,2018,38(3):363-368,6.基金项目
广东省科技计划项目(2014A020212439) （2014A020212439）
