中国耳鼻咽喉头颈外科2017，Vol.24Issue(11)：600-604,5.DOI:10.16066/j.1672-7002.2017.11.012
人β防御素2对大鼠急性鼻-鼻窦炎的抗炎防御作用研究
Study of anti-inflammatory function of human bea defensin 2 on rats' acute rhinosinusitis
摘要
Abstract
OBJECTIVE To study the antiinflammatory function of human beta defensin 2(hβD-2) on acute rhinosinusitis in rats,in order to provide a new therapy for acute rhinosinusitis.METHODS Acute rhinosinusitis model were established on SD rats before and after the transfection of plasmid,the rats in experimental group were dropped with recombinant hβD-2 plasmid mixture in nose,while with empty plasmid mixture in control group.Immunohistochemistry method was used to prove the transfection results,nasal mucosa were hematoxylineosin stained to compare the pathological difference of nasal mucosa,nasal lavage fliud was collected and cultured to compare the colony number of the bacteria.RESULTS The expression of hβD-2 was confirmed by immunohistochemistry method,which mainly distributed in mucosal epithelium and gland,pathological results showed that the inflammation of nasal mucosa in experimental group was significantly relieved than that in control group.The number of Staphylococcus auresus colony number was significantly decreased in experimental group,while there was no significantly change in the control group.CONCLUSION Recombinant hβD-2 plasmid can be successfully transfected into the nasal mucosa of rats and expressed effectively.The anti-inflammatory ability of nasal mucosa was increased after the transfection,which is expected to provide a new treatment approach for acute rhinosinusitis.关键词
β防御素/鼻窦炎/转染/抗炎Key words
beta-Defensins/Sinusitis/Transfection/anti-inflammatory引用本文复制引用
梁湘,贺广湘,闫鑫,聂尔璇,曾瑞芳,吴桐..人β防御素2对大鼠急性鼻-鼻窦炎的抗炎防御作用研究[J].中国耳鼻咽喉头颈外科,2017,24(11):600-604,5.基金项目
湖南省自然科学基金(14JJ2038)和长沙市科技局基金(K1203050-31)联合资助 （14JJ2038）
