森林与环境学报2018，Vol.38Issue(2)：156-163,8.DOI:10.13324/j.cnki.jfcf.2018.02.005
油茶种仁成熟过程油脂合成代谢的转录组分析
Transcriptomic analysis of lipid synthesis metabolism in Camellia oleifera seed kernel during its maturation period
摘要
Abstract
To explore the molecular mechanism of the oil metabolism in Camellia oleifera seed kernel,RNA-Seq was used to analysis the differential expressed genes among 3 periods of common camellia "Min 43" seed kernel during its maturation period. The results showed that the expression level of the four genes related to fatty acid and triglyceride metabolism,namely Acetyl Co-enzyme a carbox-yltransferase alpha subunit(ACCase α-CT), 3-ketoacyl-CoA synthaseⅢ(KASⅢ), long chain acyl-CoA synthetase 2 (LACS2) and glycerol-3-phosphate 2-O-acyltransferase 4(GPAT4),were up-regulated with the maturity of seed kernel.The RT-qPCR analyses were consistent with the transcriptomic data,additionally,the trend of oil content in seed kernel was in accordance with the expression level of these four genes. These findings indicate that the four genes,ACCase α-CT,KASⅢ,LACS2 and GPAT4,play important roles in the lipid metabolism of camellia seed kernel and contribute to increase the oil content of camellia seed.关键词
油茶/转录组/油脂合成代谢/差异表达基因Key words
Camellia oleifera Abel./transcriptome/lipid synthesis metabolism/differentially expressed gene分类
农业科技引用本文复制引用
吕晓杰,潘德灼,李健,陈世品,陈伟..油茶种仁成熟过程油脂合成代谢的转录组分析[J].森林与环境学报,2018,38(2):156-163,8.基金项目
国家自然科学基金项目(31070542) （31070542）
福建省种业创新与产业化工程林业项目(ZYCX-LY-2017003) （ZYCX-LY-2017003）
福建省科技重大专项(2013NZ0001-1). （2013NZ0001-1）
